| Author | Peralta, Regina H. S. | |
| Author | Melo, Daniel G. S. | |
| Author | Gonçalves, Margareth M. L. | |
| Author | D'Andrea, Paulo S. | |
| Author | Rey, Luis | |
| Author | Silva, José R. Machado | |
| Author | Peralta, José M. | |
| Access date | 2017-09-05T11:22:36Z | |
| Available date | 2017-09-05T11:22:36Z | |
| Document date | 2009 | |
| Citation | PERALTA, Regina H. S. et al. Serological Studies in Nectomys squamipes Demonstrate the Low Sensitivity of Coprological Exams for the Diagnosis of Schistosomiasis. J. Parasitol., v.95, n.3, p.764–766, 2009. | pt_BR |
| ISSN | 0022-3395 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20907 | |
| Language | eng | pt_BR |
| Publisher | American Society of Parasitologists | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Esquistossomose | pt_BR |
| Subject in Portuguese | Nectomys squamipes | pt_BR |
| Subject in Portuguese | Estudos sorológicos | pt_BR |
| Subject in Portuguese | roedores selvagens | pt_BR |
| Title | Serological studies in Nectomys squamipes demonstrate the low sensitivity of coprological exams for the diagnosis of schistosomiasis | pt_BR |
| Type | Article | |
| DOI | 10.1645/GE-1632.1 | |
| Abstract | The existence of wild rodents naturally infected by Schistosoma mansoni is a drawback for schistosomiasis control programs. As a consequence, it is necessary to have a precise diagnosis of S. mansoni infection in wild rodents (water rats; Nectomys squamipes), the species seemingly involved in the transmission of schistosomiasis at Sumindouro, Rio de Janeiro, Brazil. A total of 78 specimens of N. squamipes was captured in an endemic area at Vale do Pamparrão and Porteira Verde, Sumidouro, Brazil; 5 more were born in captivity and experimentally infected. The sensitivity and specificity of the coprological method of Kato-Katz and serological methods, i.e., enzyme-linked immunosorbent assay (ELISA) and western blot (WB), were compared. The rodents were subsequently killed and necropsied to confirm infection. The prevalences observed using ELISA (48%) and WB (41%) were equivalent to those found at necropsy (41%). The ELISA showed a sensitivity of 97% and a specificity of 87%, whereas the WB showed a sensitivity of 87% and a specificity of 89%. The Kato-Katz method exhibited 50% sensitivity and 100% specificity. The differences found among the ELISA, WB, and necropsy, when compared with Kato-Katz, may be related to the low sensitivity of the coprological method. Serological methods should be used for more reliable epidemiological information. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Serological Studies | pt_BR |
| Subject | Schistosomiasis | pt_BR |
| Subject | Nectomys squamipes | pt_BR |
| Subject | wild rodents | pt_BR |
| e-ISSN | 1937-2345 | |
| Embargo date | 2030-01-01 | |
