| Author | Fampa, Patrícia | |
| Author | Santos, André L. S. | |
| Author | Ramirez, Marcel I. | |
| Access date | 2017-09-12T16:12:35Z | |
| Available date | 2017-09-12T16:12:35Z | |
| Document date | 2010 | |
| Citation | FAMPA, Patricia; SANTOS, André L. S.; RAMIREZ, Marcel I.Trypanosoma cruzi: ubiquity expression of surface cruzipain molecules in TCI and TCII field isolates. Parasitol Res, v.107, p.443–447, 2010. | pt_BR |
| ISSN | 0932-0113 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20972 | |
| Language | eng | pt_BR |
| Publisher | Springer Verlag (Germany) | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | TCI | pt_BR |
| Subject in Portuguese | atividades de cisteína | pt_BR |
| Subject in Portuguese | atividades de metaloprotease | pt_BR |
| Subject in Portuguese | Marcadores | pt_BR |
| Subject in Portuguese | TCII | pt_BR |
| Title | Trypanosoma cruzi: ubiquity expression of surface cruzipain molecules in TCI and TCII field isolates | pt_BR |
| Type | Article | |
| DOI | 10.1007/s00436-010-1888-9 | |
| Abstract | Trypanosoma cruzi presents considerable genetic and protein profile polymorphism among different parasite populations. In a previous work, our group indicated cysteine and metalloprotease activities as good markers for separating T. cruzi I (TCI) from T. cruzi II (TCII) isolates, with higher heterogeneity among TCII isolates. Here, we have investigated the expression level of surface cruzipain in 16 field isolates belonging to the genotype TCI (n = 8) and TCII (n = 8) of T. cruzi. By means of flow cytometry analyses, using an anti-cruzipain polyclonal antibody, we observed a highly heterogeneous pattern of surface cruzipain molecules in these isolates, independently of their genotypes, cell measurements (size and granularity), original hosts, or biomes. However, fluorescence labeling tended to be stronger in TCI than in TCII population. Interestingly, isolates that expressed higher levels of surface cruzipain also yielded elevated levels of metacyclogenesis in vitro. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Integrados em Bioquímica Microbiana. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Flow cytometry analyses | pt_BR |
| Subject | metalloprotease activities | pt_BR |
| Subject | good markers | pt_BR |
| Subject | cysteine activities | pt_BR |
| e-ISSN | 1432-1955 | |
| Embargo date | 2030-01-01 | |
