| Author | Rodrigues, Luciana Silva | |
| Author | Maeda, Elisa da Silva | |
| Author | Moreira, Maria Elisabete Costa | |
| Author | Tempone, Antonio Jorge | |
| Author | Lobato, Lívia Silva | |
| Author | Resende, Victor Túlio Ribeiro | |
| Author | Alves, Lucineia | |
| Author | Rossle, Shaila | |
| Author | Lopes, Ulisses Gazos | |
| Author | Pessolani, Maria Cristina Vidal | |
| Access date | 2017-09-12T16:41:46Z | |
| Available date | 2017-09-12T16:41:46Z | |
| Document date | 2010 | |
| Citation | RODRIGUES, Luciana Silva; et al. Mycobacterium leprae induces insulin-like growth factor and promotes survival of Schwann cells upon serum withdrawal. Cellular Microbiology, v.12, n.1, p.42-54, 2010. | pt_BR |
| ISSN | 1462-5814 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/20974 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Mycobacterium leprae | pt_BR |
| Subject in Portuguese | Hanseníase | pt_BR |
| Subject in Portuguese | Células de Schwann | pt_BR |
| Subject in Portuguese | Insulin-Like Growth Factor I | pt_BR |
| Title | Mycobacterium leprae induces insulin-like growth factor and promotes survival of Schwann cells upon serum withdrawal | pt_BR |
| Type | Article | |
| DOI | 10.1111/j.1462-5822.2009.01377.x | |
| Abstract | Peripheral nerve lesions are considered the most relevant symptoms of leprosy, a chronic infectious disease caused by Mycobacterium leprae. The strategies employed by M. leprae to infect and multiply inside Schwann cells (SCs), however, remain poorly understood. In this study, it is shown that treatment of SCs with M. leprae significantly decreased cell death induced by serum deprivation. Not displayed by Mycobacterium smegmatis or Mycobacterium bovis BCG, the M. leprae survival effect was both dose dependent and specific. The conditioned medium (CM) of M. leprae-treated cultures was seen to mimic the protective effect of the bacteria, suggesting that soluble factors secreted by SCs in response to M. leprae were involved in cell survival. Indeed, by quantitative RT-PCR and dot blot/ELISA, it was demonstrated that M. leprae induced the expression and secretion of the SC survival factor insulin-like growth factor-I. Finally, the involvement of this hormone in M. leprae-induced SC survival was confirmed in experiments with neutralizing antibodies. Taken together, the results of this study delineate an important strategy for the successful colonization of M. leprae in the nerve based on the survival maintenance of the host cell through induction of IGF-I production. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Câncer. Laboratório de Medicina Experimental. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Mycobacterium leprae | pt_BR |
| Subject | Leprosy | pt_BR |
| Subject | Schwann cells | pt_BR |
| Subject | Fator de Crescimento Insulin-Like I | pt_BR |
| e-ISSN | 1462-5822 | |
| Embargo date | 2030-01-01 | |
