| Author | Guedes, Herbert Leonel de Matos | |
| Author | Pinheiro, Roberta Olmo | |
| Author | Chaves, Suzana Passos | |
| Author | De Simone, Salvatore Giovanni | |
| Author | Bergmann, Bartira Rossi | |
| Access date | 2017-09-19T14:15:26Z | |
| Available date | 2017-09-19T14:15:26Z | |
| Document date | 2010 | |
| Citation | GUEDES. Herbert Leonel de Matos; et al. Serine proteases of Leishmania amazonensis as immunomodulatory and disease-aggravating components of the crude LaAg vaccine. Vaccine, v.28, p. 5491-5496, 2010. | pt_BR |
| ISSN | 0264-410X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/21056 | |
| Language | eng | pt_BR |
| Publisher | else | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Vacina | pt_BR |
| Subject in Portuguese | Leishmaniose | pt_BR |
| Subject in Portuguese | Serina Proteases | pt_BR |
| Subject in Portuguese | Cisteína Proteases | pt_BR |
| Title | Serine proteases of Leishmania amazonensis as immunomodulatory and disease-aggravating components of the crude LaAg vaccine | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.vaccine.2010.04.109 | |
| Abstract | We previously demonstrated that intradermal and intramuscular vaccination with Leishmania amazonensis promastigote antigens (LaAg) increases the susceptibility of BALB/c mice to cutaneous leishmaniasis. In this study, we investigated the role played by serine and cysteine proteases as disease-promoting components of LaAg. Mice were immunized by the intramuscular route with LaAg that was pre-treated with a pool of serine or cysteine protease inhibitors (SPi and CPi, respectively) prior to infection with L. amazonensis. Neutralization of either enzyme type reversed the disease-promoting effect of LaAg, as seen by the slower lesion development. However, the parasite burden was only effectively controlled in mice receiving SPi-treated LaAg. Protection was associated with diminished production of TGF-beta and particularly IL-10 in response to parasite antigens by the lesion-draining lymph node cells of vaccinated mice relative to control. In vitro, soluble proteases isolated from LaAg (LaSP-Sol) directly activated IL-4, IL-10 and TGF-beta production by immune cells. Like native LaAg, vaccination with LaSP-Sol primed mice to respond to parasite challenge with a strong Jones-Mote cutaneous hypersensitivity reaction, and increased susceptibility to infection. Furthermore, neutralization of serine but not cysteine proteases blocked the capacity of LaAg to sensitize mice for Jones-Mote reaction. Together, these results indicate that soluble serine proteases are key components of LaAg responsible for its disease-promoting immunity. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular,. Laboratório de Bioquímica de Proteínas e Peptídeos,. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular,. Laboratório de Bioquímica de Proteínas e Peptídeos,. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Serine proteases | pt_BR |
| Subject | Cysteine proteases | pt_BR |
| Subject | Leishmaniasis | pt_BR |
| Subject | Leishmania amazonensis | pt_BR |
| Subject | Vacinne | pt_BR |
| e-ISSN | 1873-2518 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
