| Author | Faria, R. X. | |
| Author | Cascabulho, C. M. | |
| Author | Reis, R. A. M. | |
| Author | Alves, Luiz Anastácio | |
| Access date | 2017-09-19T17:33:52Z | |
| Available date | 2017-09-19T17:33:52Z | |
| Document date | 2010 | |
| Citation | FARIA, R. X. et al. Large-conductance channel formation mediated by P2X7 receptor activation is regulated through distinct intracellular signaling pathways in peritoneal macrophages and 2BH4 cells. Naunyn-Schmied Arch Pharmacol, v. 382, p.73–87, 2010. | pt_BR |
| ISSN | 0028-1298 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/21068 | |
| Language | eng | pt_BR |
| Publisher | Springer Verlag (Germany) | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Receptores Purinérgicos P2X7 | pt_BR |
| Subject in Portuguese | macrófagos peritoneais | pt_BR |
| Subject in Portuguese | Sistemas do Segundo Mensageiro | pt_BR |
| Subject in Portuguese | formação de poros | pt_BR |
| Title | Large-conductance channel formation mediated by P2X7 receptor activation is regulated through distinct intracellular signaling pathways in peritoneal macrophages and 2BH4 cells | pt_BR |
| Type | Article | |
| DOI | 10.1007/s00210-010-0523-8 | |
| Abstract | The P2X(7) receptor (P2X7R) is a ligand-gated ATP receptor that acts as a low- and large-conductance channel (pore) and is known to be coupled to several downstream effectors. Recently, we demonstrated that the formation of a large-conductance channel associated with the P2X(7) receptor is induced by increasing the intracellular Ca(2+) concentration (Faria et al., Am J Physiol Cell Physiol 297:C28-C42, 2005). Here, we investigated the intracellular signaling pathways associated with P2X(7) large-conductance channel formation using the patch clamp technique in conjunction with fluorescent imaging and flow cytometry assays in 2BH4 cells and peritoneal macrophages. Different antagonists were applied to investigate the following pathways: Ca(2+)-calmodulin, phospholipase A, phospholipase D, phospholipase C, protein kinase C (PKC), mitogen-activated protein kinase (MAPK), MAPK/extracellular signal-regulated kinase, phosphoinositide 3-kinase (PI3K), and cytoskeletal proteins. Macroscopic ionic currents induced by 1 mM ATP were reduced by 85% in the presence of PKC antagonists. The addition of antagonists for MAPK, PI3K, and the cytoskeleton (actin, intermediary filament, and microtubule) blocked 92%, 83%, and 95% of the ionic currents induced by 1 mM ATP, respectively. Our results show that PKC, MAPK, PI3K, and cytoskeletal components are involved in P2X(7) receptor large-channel formation in 2BH4 cells and peritoneal macrophages. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Comunicação Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Programa de Neurobiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Comunicação Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | P2X7 receptor | pt_BR |
| Subject | Intracellular Ca2+ | pt_BR |
| Subject | Pore formation | pt_BR |
| Subject | Second messenger | pt_BR |
| Subject | peritoneal macrophages | pt_BR |
| e-ISSN | 1432-1912 | |
| Embargo date | 2030-01-01 | |
