| Author | Azevedo, Z. M. A. | pt_BR |
| Author | Luz, R. A. | pt_BR |
| Author | Victal, S. H. | pt_BR |
| Author | Kurdian, B. | pt_BR |
| Author | Fonseca, V. M. | pt_BR |
| Author | Fitting, C. | pt_BR |
| Author | Câmara, F. P. | pt_BR |
| Author | Haeffner-Cavaillon, N. | pt_BR |
| Author | Cavaillon, J. -M. | pt_BR |
| Author | Elsas, Maria Ignez Capella Gaspar | pt_BR |
| Author | Xavier-Elsas, Pedro Paulo | pt_BR |
| Access date | 2010-08-23T16:00:16Z | |
| Available date | 2010-08-23T16:00:16Z | |
| Document date | 2005 | |
| Citation | AZEVEDO, Z. M. A. et al. Increased production of tumor necrosis factor-alpha in whole blood cultures from children with primary malnutrition. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, SP, v. 38, n. 2, p. 171-183, Feb. 2005. | pt_BR |
| ISSN | 0100-879X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/213 | |
| Language | eng | pt_BR |
| Rights | open access | |
| Title | Increased production of tumor necrosis factor-alpha in whole blood cultures from children with primary malnutrition | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/S0100-879X2005000200005 | |
| Abstract | Because low tumor necrosis factor-α (TNF-α) production has been reported in malnourished children, in contrast with high production of TNF-α in experimental protein-energy malnutrition, we reevaluated the production of TNF-α in whole blood cultures from children with primary malnutrition free from infection, and in healthy sex- and age- matched controls. Mononuclear cells in blood diluted 1:5 in endo- toxin-free medium released TNF-α for 24 h. Spontaneously released TNF-α levels (mean ± SEM), as measured by enzyme immunoassay in the supernatants of unstimulated 24-h cultures, were 10,941 ± 2,591 pg/ml in children with malnutrition (N = 11) and 533 ± 267 pg/ml in controls (N = 18) (P < 0.0001). TNF-α production was increased by stimulation with lipopolysaccharide (LPS), with maximal production of 67,341 ± 16,580 pg/ml TNF-α in malnourished children and 25,198 ± 2,493 pg/ml in controls (P = 0.002). In control subjects, LPS dose- dependently induced TNF-α production, with maximal responses obtained at 2000 ng/ml. In contrast, malnourished patients produced significantly more TNF-α with 0.02-200 ng/ml LPS, responded maxi- mally at a 10-fold lower LPS concentration (200 ng/ml), and presented high-dose inhibition at 2000 ng/ml. TNF-α production a) was signifi- cantly influenced by LPS concentration in control subjects, but not in malnourished children, who responded strongly to very low LPS concentrations, and b) presented a significant, negative correlation (r = -0.703, P = 0.023) between spontaneous release and the LPS concentration that elicited maximal responses in malnourished pa- tients. These findings indicate that malnourished children are not deficient in TNF-α production, and suggest that their cells are primed for increased TNF-α production. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria e Laboratório de Fisiopatologia Humana. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria e Laboratório de Fisiopatologia Humana. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria e Laboratório de Fisiopatologia Humana. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria e Laboratório de Fisiopatologia Humana. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria e Laboratório de Fisiopatologia Humana. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Institut Pasteur de Paris. Unité Cytokines et Inflammation. Paris, France. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Département de l’Évaluation Scientifique de l'INSERM. Paris, France. | |
| Affilliation | Institut Pasteur de Paris. Unité Cytokines et Inflammation. Paris, France. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Pediatria e Laboratório de Fisiopatologia Humana. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. | |
| Subject | TNF-α | |
| Subject | Malnutrition | |
| Subject | LPS | |
| DeCS | Fator de Necrose Tumoral alfa | |
| DeCS | Transtornos da Nutrição Infantil | |
| DeCS | Leucócitos Mononucleares | |
| DeCS | Lipopolissacarídeos | |
| DeCS | Células Sanguíneas | |
| DeCS | Estudos de Casos e Controles | |
| DeCS | Células Cultivadas | |
| xmlui.metadata.dc.subject.ods | 02 Fome zero e agricultura sustentável | |
