| Author | Azevedo, Ana Carolina Campi | |
| Author | Pascoal, Vanessa Peruhype Magalhães | |
| Author | Reis, Jordana Grazziela Alves Coelho dos | |
| Author | Pereira, Christiane Costa | |
| Author | Yamamura, Anna Yoshida | |
| Author | Lima, Sheila Maria Barbosa de | |
| Author | Simões, Marisol | |
| Author | Campos, Fernanda Magalhães Freire | |
| Author | Tonini, Aline de Castro Zacche | |
| Author | Lemos, Elenice Moreira | |
| Author | Brum, Ricardo Cristiano | |
| Author | Noronha, Tatiana Guimarães de | |
| Author | Freire, Marcos Silva | |
| Author | Maia, Maria de Lourdes Sousa | |
| Author | Camacho, Luiz Antônio Bastos | |
| Author | Rios, Maria | |
| Author | Chancey, Caren | |
| Author | Romano, Alessandro | |
| Author | Domingues, Carla Magda | |
| Author | Carvalho, Andréa Teixeira de | |
| Author | Martins Filho, Olindo Assis | |
| Access date | 2017-10-09T19:28:28Z | |
| Available date | 2017-10-09T19:28:28Z | |
| Document date | 2017 | |
| Citation | AZEVEDO, Ana Carolina Campi et al. Heparin removal by ecteola-cellulose pre-treatment enables the use of plasma samples for accurate measurement of anti-Yellow fever virus neutralizing antibodies. Journal of Immunological Methods, v. 448, p. 9-20, 2017. | pt_BR |
| ISSN | 0022-1759 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/22808 | |
| Language | por | pt_BR |
| Publisher | Elsevier B.V | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Dengue | pt_BR |
| Title | Heparin removal by ecteola-cellulose pre-treatment enables the use of plasma samples for accurate measurement of anti-Yellow fever virus neutralizing antibodies | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jim.2017.05.002 | |
| Abstract | Technological innovations in vaccinology have recently contributed to bring about novel insights for the vaccine-induced immune response. While the current protocols that use peripheral blood samples may provide abundant data, a range of distinct components of whole blood samples are required and the different anticoagulant systems employed may impair some properties of the biological sample and interfere with functional assays. Although the interference of heparin in functional assays for viral neutralizing antibodies such as the functional plaque-reduction neutralization test (PRNT), considered the gold-standard method to assess and monitor the protective immunity induced by the Yellow fever virus (YFV) vaccine, has been well characterized, the development of pre-analytical treatments is still required for the establishment of optimized protocols. The present study intended to optimize and evaluate the performance of pre-analytical treatment of heparin-collected blood samples with ecteola-cellulose (ECT) to provide accurate measurement of anti-YFV neutralizing antibodies, by PRNT. The study was designed in three steps, including: I. Problem statement; II. Pre-analytical steps; III. Analytical steps. Data confirmed the interference of heparin on PRNT reactivity in a dose-responsive fashion. Distinct sets of conditions for ECT pre-treatment were tested to optimize the heparin removal. The optimized protocol was pre-validated to determine the effectiveness of heparin plasma:ECT treatment to restore the PRNT titers as compared to serum samples. The validation and comparative performance was carried out by using a large range of serum vs heparin plasma:ECT 1:2 paired samples obtained from unvaccinated and 17DD-YFV primary vaccinated subjects. Altogether, the findings support the use of heparin plasma:ECT samples for accurate measurement of anti-YFV neutralizing antibodies. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecto-Parasitárias. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecto-Parasitárias. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Assessoria Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Assessoria Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Virológica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Assessoria Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | US Food and Drug Administration. Center for Biologics Evaluation and Research-CBER. Silver Spring, MD, USA. | pt_BR |
| Affilliation | US Food and Drug Administration. Center for Biologics Evaluation and Research-CBER. Silver Spring, MD, USA. | pt_BR |
| Affilliation | Ministério da Saúde. Secretaria de Vigilância em Saúde. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Ministério da Saúde. Secretaria de Vigilância em Saúde. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Ecteola-cellulose | pt_BR |
| Subject | Heparin | pt_BR |
| Subject | PRNT | pt_BR |
| Subject | Vaccinology | pt_BR |
| Subject | Yellow fever virus | pt_BR |
