| Author | Balmaseda, Angel | |
| Author | Stettler, Karin | |
| Author | Medialdea-Carrera, Raquel | |
| Author | Collado, Damaris | |
| Author | Jin, Xia | |
| Author | Zambrana, José Victor | |
| Author | Jaconi, Stefano | |
| Author | Cameroni, Elisabetta | |
| Author | Saborio, Saira | |
| Author | Rovida, Francesca | |
| Author | Percivalle, Elena | |
| Author | Ijaz, Samreen | |
| Author | Dicks, Steve | |
| Author | Ushiro-Lumb, Ines | |
| Author | Barzon, Luisa | |
| Author | Siqueira, Patricia | |
| Author | Brown, David W. G. | |
| Author | Baldanti, Fausto | |
| Author | Tedder, Richard | |
| Author | Zambon, Maria | |
| Author | Filippis, A. M. Bispo de | |
| Author | Harris, Eva | |
| Author | Corti, Davide | |
| Access date | 2017-10-10T15:20:24Z | |
| Available date | 2017-10-10T15:20:24Z | |
| Document date | 2017 | |
| Citation | BALMASEDA, Angel; et al. Antibody-based assay discriminates Zika virus infection from other flaviviruses. PNAS Early Edition, 6p, 2017. | pt_BR |
| ISSN | 1091-6490 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/22817 | |
| Language | eng | pt_BR |
| Publisher | National Academy of Sciences | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Zika | pt_BR |
| Subject in Portuguese | Dengue | pt_BR |
| Subject in Portuguese | ELISA | pt_BR |
| Subject in Portuguese | Sorologia | pt_BR |
| Subject in Portuguese | Flavivirus | pt_BR |
| Title | Antibody-based assay discriminates Zika virus infection from other flaviviruses | pt_BR |
| Type | Article | |
| DOI | 10.1073/pnas.1704984114 | |
| Abstract | Zika virus (ZIKV) is a mosquito-borne flavivirus that emerged recently as a global health threat, causing a pandemic in the Americas. ZIKV infection mostly causes mild disease, but is linked to devastating congenital birth defects and Guillain-Barré syndrome in adults. The high level of cross-reactivity among flaviviruses and their cocirculation has complicated serological approaches to differentially detect ZIKV and dengue virus (DENV) infections, accentuating the urgent need for a specific and sensitive serological test. We previously generated a ZIKV nonstructural protein 1 (NS1)-specific human monoclonal antibody, which we used to develop an NS1-based competition ELISA. Well-characterized samples from RT-PCR-confirmed patients with Zika and individuals exposed to other flavivirus infections or vaccination were used in a comprehensive analysis to determine the sensitivity and specificity of the NS1 blockade-of-binding (BOB) assay, which was established in laboratories in five countries (Nicaragua, Brazil, Italy, United Kingdom, and Switzerland). Of 158 sera/plasma from RT-PCR-confirmed ZIKV infections, 145 (91.8%) yielded greater than 50% inhibition. Of 171 patients with primary or secondary DENV infections, 152 (88.9%) scored negative. When the control group was extended to patients infected by other flaviviruses, other viruses, or healthy donors (n = 540), the specificity was 95.9%. We also analyzed longitudinal samples from DENV-immune and DENV-naive ZIKV infections and found inhibition was achieved within 10 d postonset of illness and maintained over time. Thus, the Zika NS1 BOB assay is sensitive, specific, robust, simple, low-cost, and accessible, and can detect recent and past ZIKV infections for surveillance, seroprevalence studies, and intervention trials. | pt_BR |
| Affilliation | Ministry of Health. Centro Nacional de Diagnóstico y Referencia. Laboratorio Nacional de Virología. Managua, Nicaragua / .Sustainable Sciences Institute. Managua 14007, Nicaragua. | pt_BR |
| Affilliation | Humabs BioMed SA, 6500 Bellinzona, Switzerland. | pt_BR |
| Affilliation | University of Liverpool. National Institute for Health Research Health Protection Research Unit in Emerging and Zoonotic Infections. Liverpool, United Kingdom. | pt_BR |
| Affilliation | Sustainable Sciences Institute. Managua 14007, Nicaragua. | pt_BR |
| Affilliation | Humabs BioMed SA, 6500 Bellinzona, Switzerland. | pt_BR |
| Affilliation | Sustainable Sciences Institute. Managua 14007, Nicaragua. | pt_BR |
| Affilliation | Humabs BioMed SA, 6500 Bellinzona, Switzerland. | pt_BR |
| Affilliation | Humabs BioMed SA, 6500 Bellinzona, Switzerland. | pt_BR |
| Affilliation | Ministry of Health. Centro Nacional de Diagnóstico y Referencia. Laboratorio Nacional de Virología. Managua, Nicaragua / .Sustainable Sciences Institute. Managua 14007, Nicaragua. | pt_BR |
| Affilliation | Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo. Molecular Virology Unit, Microbiology and Virology Department. Pavia, Italy. | pt_BR |
| Affilliation | Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo. Molecular Virology Unit, Microbiology and Virology Department. Pavia, Italy. | pt_BR |
| Affilliation | Public Health England. Microbiology Services Colindale. Londo, United Kingdom. | pt_BR |
| Affilliation | Public Health England. Microbiology Services Colindale. Londo, United Kingdom. | pt_BR |
| Affilliation | Microbiology Services. National Health Service Blood and Transplant. London, United Kingdom. | pt_BR |
| Affilliation | University of Padova. Department of Molecular Medicine. Padova, Italy. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo. Molecular Virology Unit, Microbiology and Virology Department. Pavia, Italy / University of Pavia. Department of Clinical, Surgical, Diagnostic, and Pediatric Sciences. Pavia, Italy. | pt_BR |
| Affilliation | Public Health England. Microbiology Services Colindale. Londo, United Kingdom. | pt_BR |
| Affilliation | Public Health England. Microbiology Services Colindale. Londo, United Kingdom. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | University of California. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, CA, USA. | pt_BR |
| Affilliation | Humabs BioMed SA, 6500 Bellinzona, Switzerland. | pt_BR |
| Subject | Zika | pt_BR |
| Subject | serology | pt_BR |
| Subject | flaviruses | pt_BR |
| Subject | dengue | pt_BR |
| Subject | ELISA | pt_BR |
