| Author | Santos, Jefferson José da Silva | |
| Author | Magalhães, Tereza | |
| Author | Silva Junior, José Valter Joaquim | |
| Author | Silva, Andréa Nazaré Monteiro Rangel da | |
| Author | Cordeiro, Marli Tenório | |
| Author | Gil, Laura Helena Vega Gonzales | |
| Access date | 2017-10-13T12:01:09Z | |
| Available date | 2017-10-13T12:01:09Z | |
| Document date | 2015 | |
| Citation | SANTOS, J. J. DA S. et al. Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil. Memorias Do Instituto Oswaldo Cruz, v. 110, n. 5, p. 677–683, ago. 2015. | pt_BR |
| ISSN | 1678-8060 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/22828 | |
| Language | por | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Vírus da Dengue | pt_BR |
| Subject in Portuguese | DNA Complementar | pt_BR |
| Subject in Portuguese | RNA Viral | pt_BR |
| Title | Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/0074-02760150053 | |
| Abstract | Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil / Departamento de Saúde do Estado de Pernambuco, Laboratório Central de Saúde Pública. Recife, PE, Brasil | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil. | |
| Subject | Brazil | pt_BR |
| Subject | Cloning, Molecular | pt_BR |
| Subject | Dengue Virus | pt_BR |
| Subject | DNA, Complementary | pt_BR |
| Subject | Molecular Sequence Data | pt_BR |
| Subject | RNA, Viral | pt_BR |
| Subject | Sequence Analysis, DNA | pt_BR |
| Subject | Virus Replication | pt_BR |
| DeCS | Brasil | pt_BR |
| DeCS | Vírus da dengue | pt_BR |
| DeCS | DNA Complementar | pt_BR |
| DeCS | RNA Viral | pt_BR |
| DeCS | Clonagem molecular | pt_BR |
| DeCS | Dados de Sequência Molecular | pt_BR |
| DeCS | Replicação Viral | pt_BR |
