| Author | Mendes, Ricardo E. | |
| Author | Zafra, Rafael | |
| Author | Pérez-Écija, Rafael A. | |
| Author | Buffoni, Leandro | |
| Author | Martínez-Moreno, Álvaro | |
| Author | Tendler, Miriam | |
| Author | Pérez, José | |
| Access date | 2017-10-19T11:04:06Z | |
| Available date | 2017-10-19T11:04:06Z | |
| Document date | 2010 | |
| Citation | MENDES, Ricardo E. et al. Evaluation of local immune response to Fasciola hepatica experimental infection in the liver and hepatic lymph nodes of goats immunized with Sm14 vaccine antigen. Mem Inst Oswaldo Cruz, v.105, n.5, p.698-705, Aug. 2010. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/22877 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Cabras | pt_BR |
| Subject in Portuguese | infecção parasitária | pt_BR |
| Subject in Portuguese | Vacinas | pt_BR |
| Subject in Portuguese | Fasciola hepatica | pt_BR |
| Subject in Portuguese | Fígado | pt_BR |
| Title | Evaluation of local immune response to Fasciola hepatica experimental infection in the liver and hepatic lymph nodes of goats immunized with Sm14 vaccine antigen | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762010000500017 | |
| Abstract | Protection against Fasciola hepatica in goats immunized with a synthetic recombinant antigen from Schistosoma mansoni fatty acid-binding protein 14 (rSm14) was investigated by assessing worm burdens, serum levels of hepatic enzymes, faecal egg count and hepatic damage, which was evaluated using gross and microscopic morphometric observation. The nature of the local immune response was assessed by examining the distribution of CD2+, CD4+, CD8+ and γδ+ T lymphocytes along with IgG+, IL-4+ and IFN-γ+ cells in the liver and hepatic lymph nodes (HLN). The goats used consisted of group 1 (unimmunized and uninfected), group 2 [infected control - immunized with Quillaia A (Quil A)] and group 3 (immunized with rSm14 in Quil A and infected), each containing seven animals. Immunization with rSm14 in Quil A adjuvant induced a reduction in gross hepatic lesions of 56.6% (p < 0.001) and reduced hepatic and HLN infiltration of CD2+, CD4+, CD8+ and γδ+ T lymphocytes as well as IL-4+ and IFN-γ+ cells (p < 0.05). This is the first report of caprine immunization against F. hepatica using a complete rSm14 molecule derived from S. mansoni. Immunization reduced hepatic damage and local inflammatory infiltration into the liver and HLN. However, considering that Quil A is not the preferential/first choice adjuvant for Sm14 immunization, further studies will be undertaken using the monophosphoryl lipid A-based family of adjuvants during clinical trials to facilitate anti-fasciola vaccine development. | pt_BR |
| Affilliation | Universidad de Córdoba. Facultad de Veterinaria. Departamento de Anatomía y Anatomía Patológica Comparadas.Córodba, Spain. | pt_BR |
| Affilliation | Universidad de Córdoba. Facultad de Veterinaria. Departamento de Anatomía y Anatomía Patológica Comparadas.Córodba, Spain. | pt_BR |
| Affilliation | Universidad de Córdoba. Facultad de Veterinaria. Departamento de Anatomía y Anatomía Patológica Comparadas.Córodba, Spain. | pt_BR |
| Affilliation | Universidad de Córdoba. Facultad de Veterinaria. Departamento de Sanidad Animal (Parasitologia). Córodoba, Spain. | pt_BR |
| Affilliation | Universidad de Córdoba. Facultad de Veterinaria. Departamento de Sanidad Animal (Parasitologia). Córodoba, Spain. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidad de Córdoba. Facultad de Veterinaria. Departamento de Anatomía y Anatomía Patológica Comparadas.Córodba, Spain. | pt_BR |
| Subject | Sm14 | pt_BR |
| Subject | Fasciola hepatica | pt_BR |
| Subject | vaccines | pt_BR |
| Subject | goat | pt_BR |
| Subject | liver | pt_BR |
| Subject | parasite infection | pt_BR |
| e-ISSN | 1678-8060 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
