| Author | Coutinho, Carlos José P. C. Araújo | |
| Author | Bernardino, Thaissa C. | |
| Author | Pires, Eder S. | |
| Author | Esch, Lygia V. S. | |
| Author | Viviani, Andrea B. P. | |
| Author | Cavados, Clara F. G. | |
| Access date | 2017-10-19T12:11:29Z | |
| Available date | 2017-10-19T12:11:29Z | |
| Document date | 2011 | |
| Citation | COUTINHO, Carlos José P. C. Araújo; et al. Investigation of biota associated and natural infection by sporulated bacteria in Culicidae larvae from São Paulo state, Brazil. Journal of Invertebrate Pathology, v.107, p.11–15, 2011. | pt_BR |
| ISSN | 0022-2011 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/22880 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Culicidae | pt_BR |
| Subject in Portuguese | Larva | pt_BR |
| Subject in Portuguese | biodiversidade bacteriana | pt_BR |
| Subject in Portuguese | Bacillus thuringiensis | pt_BR |
| Subject in Portuguese | Bacillus cereus | pt_BR |
| Title | Investigation of biota associated and natural infection by sporulated bacteria in Culicidae larvae from São Paulo state, Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jip.2011.01.003 | |
| Abstract | The aim of this study was to investigate the naturally occurring bacteria from Culicidae larvae found in São Paulo state between 2006 and 2008 from the Tiete Ecological Park in the municipality of Caraguatatuba. Bacterial strains were obtained after surface sterilization of larvae followed by thermal treatment and incubation in bacteriological media under laboratory conditions. Identification was determined using cytomorphology, biochemical and physiological tests. Strains were characterized by qualitative evaluation of biological activity against Culicidae larvae, comparing protein profiles obtained by electrophoresis of crystal protoxins, electrophoresis of Lysinibacillus sphaericus isoenzymes, detection of the L(2) fraction of Hemolytic BL enterotoxin and amplification of DNA using Multiplex-PCR to detect HBL, NHE, CytK, BceT and EntFM enterotoxins. The results obtained in this study demonstrated that the endosymbiotic bacterial diversity belonging to Bacillus and related genera associated with Culicidae larvae is limited to a number of species that does not vary throughout the seasons. | pt_BR |
| Affilliation | Instituto Butantan. Laboratório de Imunologia Viral. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Instituto Butantan. Laboratório de Imunologia Viral. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Superintendência de Controle de Endemias. Laboratório de Simulídeos. Caraguatatuba, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Bacillus thuringiensis | pt_BR |
| Subject | Bacillus cereus | pt_BR |
| Subject | Lysinibacillus sphaericus | pt_BR |
| Subject | Culicidae larvae and bacterial biodiversity | pt_BR |
| e-ISSN | 1096-0805 | |
| Embargo date | 2030-01-01 | |
| xmlui.metadata.dc.subject.ods | 15 Vida terrestre | |
