| Autor | Reed, Steven G | |
| Autor | Carvalho, Edgar Marcelino | |
| Autor | Sherbert, Cynthia H | |
| Autor | Sampaio, Diana Pedral | |
| Autor | Russo, Donna M | |
| Autor | Bacelart, Olivia | |
| Autor | Pihl, Deanna L | |
| Autor | Jeannine M. Scott | |
| Autor | Barral, Aldina Maria Prado | |
| Autor | Grabstein, Kenneth H | |
| Autor | Johnson Junior, Warren D | |
| Data de acesso | 2017-10-27T18:51:32Z | |
| Data de disponibilização | 2017-10-27T18:51:32Z | |
| Data do publicação | 1990 | |
| Citação | REED, S. G. et al. In Vitro Responses to Leishmania Antigens by Lymphocytes from Patients with Leishmaniasis or Chagas' Disease. Journal of Clinical Investigation, v. 85, p. 690-696, 1990. | pt_BR |
| ISSN | 0021-9738 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/22978 | |
| Fomento | AI-25038 and AI-16282 from the National Institutes of Health and by the UNDP/World Bank/World Health Organization | pt_BR |
| Idioma | eng | pt_BR |
| Editor | American Society for Clinical Investigation | pt_BR |
| Direito Autoral | open access | pt_BR |
| Palavras-chave | Leishmania | pt_BR |
| Palavras-chave | Antígenos | pt_BR |
| Palavras-chave | Células T | pt_BR |
| Palavras-chave | Leishmaniose | pt_BR |
| Título | In Vitro Responses to Leishmania Antigens by Lymphocytes from Patients with Leishmaniasis or Chagas' Disease | pt_BR |
| Tipo do documento | Article | pt_BR |
| Resumo em Inglês | T cell responses are correlated with recovery from and resistance
to leishmaniasis. Antigens of Leishmania chagasi were
evaluated by determining their ability to elicit in vitro proliferation
and cytokine production in peripheral blood lymphocytes
and in T cell lines and clones from patients with histories of
leishmaniasis or Chagas' disease. Antigens tested were selected
by their reactivity with patient antibodies. Several of the
antigens induced proliferative responses in peripheral blood
lymphocytes from patients recovered from visceral or cutaneous
leishmaniasis or with chronic Chagas' disease. Two purified
glycoproteins, 30 and 42 kD, were consistently among the
most effective in eliciting high proliferative responses and IL-2
production. Lymphocytes from a recovered visceral leishmaniasis
patient were used to produce T cell lines against either the
30- or 42-kD antigen. Each of the lines responded to both of
these antigens as well as to crude leishmania lysate. Cb4+ T
cell clones specific for either or both of these antigens were
also isolated from a visceral leishmaniasis patient. In contrast,
rabbit antisera produced against these two antigens were not
crossreactive. Both antigens were effective in inducing the production
of IFN-'y from T cell lines from both leishmaniasis and
Chagas' disease patients. These studies demonstrate the potential
for defining parasite antigens with broad immunostimulatory
capabilities. | pt_BR |
| Afiliação | Seattle Biomedical Research Institute. Seattle, Washington | pt_BR |
| Afiliação | Federal University of Bahia. Hospitalar Prof. Edgard Santos. Salvador, BA, Brazil | pt_BR |
| Afiliação | Seattle Biomedical Research Institute. Seattle, Washington | pt_BR |
| Afiliação | Federal University of Bahia. Hospitalar Prof. Edgard Santos. Salvador, BA, Brazil | pt_BR |
| Afiliação | Seattle Biomedical Research Institute. Seattle, Washington | pt_BR |
| Afiliação | Federal University of Bahia. Hospitalar Prof. Edgard Santos. Salvador, BA, Brazil | pt_BR |
| Afiliação | Seattle Biomedical Research Institute. Seattle, Washington | pt_BR |
| Afiliação | Seattle Biomedical Research Institute. Seattle, Washington | pt_BR |
| Afiliação | Federal University of Bahia. Hospitalar Prof. Edgard Santos. Salvador, BA, Brazil | pt_BR |
| Afiliação | Immunex Corporation. Seattle, Washington | pt_BR |
| Afiliação | Cornell University Medical College. New York, New York | pt_BR |
| Palavras-chave em inglês | Leishmania | pt_BR |
| Palavras-chave em inglês | Antigens | pt_BR |
| Palavras-chave em inglês | T cells | pt_BR |
| Palavras-chave em inglês | Leishmaniasis | pt_BR |
