| Author | Chalegre, Karlos Diogo de Melo | |
| Author | Tavares, Daniella A. | |
| Author | Romão, Tatiany P. | |
| Author | de Menezes, Heverly Suzany G. | |
| Author | Nascimento, Nathaly A. | |
| Author | de Oliveira, Cláudia Maria F. | |
| Author | de-Melo-Neto, Osvaldo P. | |
| Author | Silva-Filha, Maria Helena N. L. | |
| Access date | 2017-11-14T11:54:28Z | |
| Available date | 2017-11-14T11:54:28Z | |
| Document date | 2017 | |
| ISSN | 1742-4658 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/23149 | |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| MeSH | Bacillaceae | pt_BR |
| MeSH | patogenicidade | pt_BR |
| MeSH | Culex | pt_BR |
| MeSH | genética | pt_BR |
| MeSH | Culex | pt_BR |
| MeSH | microbiologia | pt_BR |
| MeSH | Animais Toxinas Bacterianas | pt_BR |
| MeSH | metabolismo | pt_BR |
| MeSH | Toxinas Bacterianas | pt_BR |
| MeSH | toxicidade | pt_BR |
| MeSH | Cruzamentos Genéticos Culex | pt_BR |
| MeSH | enzimologia | pt_BR |
| MeSH | Evolução Molecular | pt_BR |
| MeSH | Feminino | pt_BR |
| MeSH | Frequência do Gene | pt_BR |
| MeSH | Genes de Insetos | pt_BR |
| MeSH | Infecções por Bactérias Gram-Positivas | pt_BR |
| MeSH | Proteínas de Insetos | pt_BR |
| MeSH | metabolismo | pt_BR |
| MeSH | Larva | pt_BR |
| MeSH | enzimologia | pt_BR |
| MeSH | microbiologia | pt_BR |
| MeSH | Masculino | pt_BR |
| MeSH | Mutação | pt_BR |
| MeSH | Seleção | pt_BR |
| MeSH | Genética alfa-Glucosidases | pt_BR |
| MeSH | alfa-Glucosidases | pt_BR |
| Subject in Portuguese | Bacillaceae | pt_BR |
| Subject in Portuguese | Culex | pt_BR |
| Title | Co-selection and replacement of resistance alleles to Lysinibacillus sphaericus in a Culex quinquefasciatus colony | pt_BR |
| Type | Article | |
| Abstract | The Cqm1 α-glucosidase, expressed within the midgut of Culex quinquefasciatus mosquito larvae, is the receptor for the Binary toxin (Bin) from the entomopathogen Lysinibacillus sphaericus. Mutations of the Cqm1 α-glucosidase gene cause high resistance levels to this bacterium in both field and laboratory populations, and a previously described allele, cqm1REC, was found to be associated with a laboratory-resistant colony (R2362). This study described the identification of a novel resistance allele, cqm1REC-2, that was co-selected with cqm1REC within the R2362 colony. The two alleles display distinct mutations but both generate premature stop codons that prevent the expression of midgut-bound Cqm1 proteins. Using a PCR-based assay to monitor the frequency of each allele during long-term maintenance of the resistant colony, cqm1REC was found to predominate early on but later was replaced by cqm1REC-2 as the most abundant resistance allele. Homozygous larvae for each allele were then generated that displayed similar high-resistance phenotypes with equivalent low levels of transcript and lack of protein expression for both cqm1REC and cqm1REC-2. In progeny from a cross of homozygous individuals for each allele at a 1 : 1 ratio, analyzed for ten subsequent generations, cqm1REC showed a higher frequency than cqm1REC-2. The replacement of cqm1REC by cqm1REC -2 observed in the R2362 colony, kept for 210 generations, indicates changes in fitness related to traits that are unknown but linked to these two alleles, and constitutes a unique example of evolution of resistance within a controlled laboratory environment. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil | pt_BR |
| Subject | Bacillaceae | pt_BR |
| Subject | allele replacement | pt_BR |
| Subject | alpha-Glucosidases | pt_BR |
| Subject | Animals | pt_BR |
| Subject | Bacillaceae | pt_BR |
| Subject | Bacterial Toxins | pt_BR |
| Subject | cqm1 alleles | pt_BR |
| Subject | Crosses, Genetic | pt_BR |
| Subject | Culex | pt_BR |
| Subject | Evolution, Molecular | pt_BR |
| Subject | Gene Frequency | pt_BR |
| Subject | Genes, Insect | pt_BR |
| Subject | Gram-Positive Bacterial Infections | pt_BR |
| Subject | Insect Proteins | pt_BR |
| Subject | Male | pt_BR |
| Subject | Mutation | pt_BR |
| Subject | receptors | pt_BR |
| Subject | Selection, Genetic | pt_BR |
| Subject | alpha-glucosidases | pt_BR |
| e-ISSN | 10.1111/febs.13364 | |
