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AutorOliveira, Valéria da Costa
AutorBoechat, Viviane Cardoso
AutorMendes Junior, Artur Augusto Velho
AutorMadeira, Maria de Fátima
AutorFerreira, Luiz Claudio
AutorFigueiredo, Fabiano Borges
AutorCampos, Monique Paiva
AutorRodrigues, Francisco das Chagas de Carvalho
AutorOliveira, Raquel de Vasconcellos Carvalhaes de
AutorAmendoeira, Maria Regina Reis
AutorMenezes, Rodrigo Caldas
Fecha de acceso2017-11-16T11:25:00Z
Fecha de disponibilización2017-11-16T11:25:00Z
Fecha de publicación2017
ReferenciaOLIVEIRA, Valéria da Costa; et al. Occurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterations. Plos One, v.12, n.4, e0175588, 15p, Apr. 2017.pt_BR
ISSN1932-6203pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/23197
Idiomaengpt_BR
EditorPublic Library of Sciencept_BR
Derechos de autoropen access
Palabras clave en PortuguésLeishmania infantumpt_BR
Palabras clave en PortuguésZoonosespt_BR
Palabras clave en PortuguésLeishmaniose Visceralpt_BR
Palabras clave en PortuguésSistema Nervoso Centralpt_BR
TítuloOccurrence of Leishmania infantum in the central nervous system of naturally infected dogs: Parasite load, viability, co-infections and histological alterationspt_BR
Tipo del documentoArticle
DOI10.1371/journal.pone.0175588
Resumen en InglésZoonotic visceral leishmaniasis is caused by the protozoan Leishmania infantum and little is known about the occurrence and pathogenesis of this parasite in the CNS. The aims of this study were to evaluate the occurrence, viability and load of L. infantum in the CNS, and to identify the neurological histological alterations associated with this protozoan and its co-infections in naturally infected dogs. Forty-eight Leishmania-seropositive dogs from which L. infantum was isolated after necropsy were examined. Cerebrospinal fluid (CSF) samples were analyzed by parasitological culture, quantitative real-time PCR (qPCR) and the rapid immunochromatographic Dual Path Platform test. Brain, spinal cord and spleen samples were submitted to parasitological culture, qPCR, and histological techniques. Additionally, anti-Toxoplasma gondii and anti-Ehrlichia canis antibodies in serum and distemper virus antigens in CSF were investigated. None of the dogs showed neurological signs. All dogs tested positive for L. infantum in the CNS. Viable forms of L. infantum were isolated from CSF, brain and spinal cord in 25% of the dogs. Anti-L. infantum antibodies were detected in CSF in 61% of 36 dogs. Inflammatory histological alterations were observed in the CNS of 31% of the animals; of these, 66% were seropositive for E. canis and/or T. gondii. Amastigote forms were associated with granulomatous non-suppurative encephalomyelitis in a dog without evidence of co-infections. The highest frequency of L. infantum DNA was observed in the brain (98%), followed by the spinal cord (96%), spleen (95%), and CSF (50%). The highest L. infantum load in CNS was found in the spinal cord. These results demonstrate that L. infantum can cross the blood-brain barrier, spread through CSF, and cause active infection in the entire CNS of dogs. Additionally, L. infantum can cause inflammation in the CNS that can lead to neurological signs with progression of the disease.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Serviço de Anatomia Patológica. Rio de Janeiro, RJ, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Serviço de Anatomia Patológica. Rio de Janeiro, RJ, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Epidemiologia Clínica. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ. Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evando Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ. Brasil.pt_BR
Palavras clave en InglêsLeishmania infantumpt_BR
Palavras clave en Inglêscentral nervous systempt_BR
Palavras clave en InglêsZoonotic visceral leishmaniasispt_BR


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