| Author | Chaves, Lana Bitencourt | |
| Author | Silva, Daiana de Souza Perce da | |
| Author | Silva, Rodrigo Nunes Rodrigues da | |
| Author | Silva, João Hermínio Martins da | |
| Author | Cassiano, Gustavo Capatti | |
| Author | Machado, Ricardo Luiz Dantas | |
| Author | Riccio, Lilian Rose Pratt | |
| Author | Banic, Dalma Maria | |
| Author | Lima Junior, Josué da Costa | |
| Access date | 2017-11-21T10:18:00Z | |
| Available date | 2017-11-21T10:18:00Z | |
| Document date | 2017 | |
| Citation | CHAVES, Lana Bitencourt et al. Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon. PLoS Neglected Tropical Diseases, v. 11, n. 2, p. 1-19, Feb. 2017. | pt_BR |
| ISSN | 1935-2727 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/23276 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Plasmodium vivax | pt_BR |
| Subject in Portuguese | Esporozoítos | pt_BR |
| Subject in Portuguese | Amazônia Brasileira | pt_BR |
| Subject in Portuguese | Epitopos de Linfócito B | pt_BR |
| Subject in Portuguese | Ookinetes | pt_BR |
| Subject in Portuguese | Malária | pt_BR |
| Title | Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pntd.0005344 | |
| Abstract | The Plasmodium vivax Cell-traversal protein for ookinetes and sporozoites (PvCelTOS) plays an important role in the traversal of host cells. Although essential to PvCelTOS progress as a vaccine candidate, its genetic diversity remains uncharted. Therefore, we investigated the PvCelTOS genetic polymorphism in 119 field isolates from five different regions of Brazilian Amazon (Manaus, Novo Repartimento, Porto Velho, Plácido de Castro and Oiapoque). Moreover, we also evaluated the potential impact of non-synonymous mutations found in the predicted structure and epitopes of PvCelTOS. The field isolates showed high similarity (99.3% of bp) with the reference Sal-1 strain, presenting only four Single-Nucleotide Polymorphisms (SNP) at positions 24A, 28A, 109A and 352C. The frequency of synonymous C109A (82%) was higher than all others (p<0.0001). However, the non-synonymous G28A and G352C were observed in 9.2% and 11.7% isolates. The great majority of the isolates (79.8%) revealed complete amino acid sequence homology with Sal-1, 10.9% presented complete homology with Brazil I and two undescribed PvCelTOS sequences were observed in 9.2% field isolates. Concerning the prediction analysis, the N-terminal substitution (Gly10Ser) was predicted to be within a B-cell epitope (PvCelTOS Accession Nos. AB194053.1) and exposed at the protein surface, while the Val118Leu substitution was not a predicted epitope. Therefore, our data suggest that although G28A SNP might interfere in potential B-cell epitopes at PvCelTOS N-terminal region the gene sequence is highly conserved among the isolates from different geographic regions, which is an important feature to be taken into account when evaluating its potential as a vaccine candidate. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Ceará. Grupo de Modelagem Computacional. Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Campinas. Departamento de Genética, Evolução e Bioagentes. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. Laboratório de Imunogenética da Malária. Belém, PA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Plasmodium vivax | pt_BR |
| Subject | Sporozoites | pt_BR |
| Subject | Brazilian Amazon | pt_BR |
| Subject | potential B-cell epitopes | pt_BR |
| Subject | ookinetes | pt_BR |
| Subject | Malaria | pt_BR |
| e-ISSN | 1935-2735 | |
