| Author | Silva Jr., J. V. J. | |
| Author | Arenhart, S. | |
| Author | Santos, H. F. | |
| Author | Almeida-Queiroz, S. R. | |
| Author | Silva, A. N. M. R. | |
| Author | Trevisol, I. M. | |
| Author | Bertani, G. R. | |
| Author | Gil, L. H. V. G. | |
| Access date | 2017-11-30T13:15:29Z | |
| Available date | 2017-11-30T13:15:29Z | |
| Document date | 2014 | |
| Citation | SILVA JR., J. V. J. et al. Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast. Brazilian Journal of Microbiology, v. 45, n. 4, p. 1555–1563, dez. 2014. | pt_BR |
| ISSN | 1678-4405 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/23468 | |
| Language | eng | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Vírus da Doença Infecciosa da Bursa | pt_BR |
| Subject in Portuguese | Genética Reversa | pt_BR |
| Subject in Portuguese | recombinação homóloga baseada em fermento | pt_BR |
| Title | Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/S1517-83822014000400054 | |
| Abstract | The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro ligation and restriction sites. The aim of this research was to build the world's first VRG for IBDV by yeast-based homologous recombination; a more efficient, robust and simple process than cloning by in vitro ligation. The wild type IBDV (Wt-IBDV-Br) was isolated in Brazil and had its genome cloned in pJG-CMV-HDR vector by yeast-based homologous recombination. The clones were transfected into chicken embryo fibroblasts and the recovered virus (IC-IBDV-Br) showed genetic stability and similar phenotype to Wt-IBDV-Br, which were observed by nucleotide sequence, focus size/morphology and replication kinetics, respectively. Thus, IBDV reverse genetics by yeast-based homologous recombination provides tools to IBDV understanding and vaccines/viral vectors development. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil / Universidade Federal de Santa Maria. Centro de Ciências Rurais. Setor de Virologia. Departamento de Medicina Veterinária Preventiva. Santa Maria, RS, Brazil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Instituto de Ciências Básicas da Saúde. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratório de Virologia. Porto Alegre, RS, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil. | pt_BR |
| Affilliation | Embrapa Suínos e Aves . Concórdia, SC, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Pernambuco. Departamento de Bioquímica e Laboratório de Imunopatologia Keiso Asami. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil. | pt_BR |
| Subject | infectious bursal disease virus | pt_BR |
| Subject | reverse genetics | pt_BR |
| Subject | yeast-based homologous recombination | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Embrião de Galinha | pt_BR |
| DeCS | Recombinação Homóloga | pt_BR |
| DeCS | Vetores Genéticos | pt_BR |
| DeCS | Vírus da Doença Infecciosa da Bursa | pt_BR |
| DeCS | genética | pt_BR |
| DeCS | Genética Reversa | pt_BR |
| DeCS | métodos | pt_BR |
| DeCS | Brasil | pt_BR |
| DeCS | Células Cultivadas | pt_BR |
| DeCS | Fibroblastos | pt_BR |
| DeCS | virologia | pt_BR |
| DeCS | Instabilidade Genômica | pt_BR |
| DeCS | Transfecção | pt_BR |
| DeCS | Vírus da Doença Infecciosa da Bursa | pt_BR |
| DeCS | isolamento & purificação | pt_BR |
| DeCS | Vírus da Doença Infecciosa da Bursa | pt_BR |
| DeCS | fisiologia | pt_BR |
| DeCS | Saccharomyces cerevisiae | pt_BR |
| DeCS | genética | pt_BR |
| DeCS | Cultura de Vírus | pt_BR |
| DeCS | Replicação Viral | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
