Author | Nogueira, Caio Cesar Richter | |
Author | Paixão, Izabel Christina Nunes de Palmer | |
Author | Santos, Claudio Cesar Cirne | |
Author | Stephens, Paulo Roberto Soares | |
Author | Villaça, Roberto Campos | |
Author | Pereira, Helena de Souza | |
Author | Teixeira, Valéria Laneuville | |
Access date | 2017-12-21T16:04:33Z | |
Available date | 2017-12-21T16:04:33Z | |
Document date | 2016 | |
Citation | NOGUEIRA, Caio Cesar Richter; et al. Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera. Journal of Medicinal Plants Research, v.10, n.35, p.621-625, Sept. 2016. | pt_BR |
ISSN | 1996-0875 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/23745 | |
Language | eng | pt_BR |
Publisher | Academic Journals | pt_BR |
Rights | open access | |
Subject in Portuguese | HIV-1 | pt_BR |
Subject in Portuguese | Acanthophora spicifera | pt_BR |
Subject in Portuguese | Alga Marinha vermelha | pt_BR |
Subject in Portuguese | Atividade antiviral | pt_BR |
Title | Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera | pt_BR |
Type | Article | |
DOI | 10.5897/JMPR2016.6208 | |
Abstract | First generation drugs such as zidovudine have been extensively used in clinical practice, resulting in
the development of HIV resistance to these nucleoside analogs. Several studies have demonstrated the
effective anti-HIV activity of natural products derived from seaweeds, suggesting promising sources of
substances for the development of novel antiviral drugs. In this paper, the antiviral effect of extracts
from the red seaweed Acanthophora spicifera on HIV-1 replication was evaluated in vitro. Peripheral
blood mononuclear cells obtained using the Ficoll-Hypaque gradient were used for cytotoxicity and
antiviral activity testing. The dichloromethane extracts, ethyl acetate, acetone, and methanol were
found to have CC50 values of 31±7.4, 45±11, 38±3.5, and 179±25 μg/mL, respectively. With the control,
the extract prepared in ethyl acetate inhibited approximately 60% of the viral load, which is the best
result among the extracts. This same extract showed an IC50 value of 33.17±4.84 μg/mL for the reverse
transcriptase. The EtOAc extract from A. spicifera showed to be an efficient HIV antiviral due to its
phenolic compounds, as evaluated by nuclear magnetic resonance. | pt_BR |
Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil. | pt_BR |
Subject | Marine natural products | pt_BR |
Subject | red seaweed | pt_BR |
Subject | Acanthophora spicifera | pt_BR |
Subject | HIV-1 | pt_BR |
Subject | Antiviral activity | pt_BR |
Subject | Anti-HIV-1 RT | pt_BR |