| Author | Dalla Costa, Elis R. | |
| Author | Vasconcelos, Sidra E. G. | |
| Author | Esteves, Leonardo S. | |
| Author | Gomes, Harrison M. | |
| Author | Gomes, Lia L. | |
| Author | Silva, Pedro Almeida da | |
| Author | Perdigão, João | |
| Author | Portugal, Isabel | |
| Author | Viveiros, Miguel | |
| Author | McNerney, Ruth | |
| Author | Pain, Arnab | |
| Author | Clark, Taane G. | |
| Author | Rastogi, Nalin | |
| Author | Unis, Gisela | |
| Author | Rossetti, Maria Lucia R. | |
| Author | Suffys, Philip Noel | |
| Access date | 2018-01-02T16:05:14Z | |
| Available date | 2018-01-02T16:05:14Z | |
| Document date | 2015 | |
| Citation | DALLA COSTA, Elis R. et al. Multidrug-Resistant Mycobacterium tuberculosis of the Latin American Mediterranean Lineage, Wrongly Identified as Mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), Causing Active Tuberculosis in South Brazil. Journal of Clinical Microbiology, v.53, n.12, p.3805-3811, Dec. 2015. | pt_BR |
| ISSN | 0095-1137 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/23846 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Tuberculose | pt_BR |
| Subject in Portuguese | Mycobacterium tuberculosis | pt_BR |
| Subject in Portuguese | Resistência a medicamentos | pt_BR |
| Subject in Portuguese | Mycobacterium pinnipedii | pt_BR |
| Subject in Portuguese | América Latina | pt_BR |
| Subject in Portuguese | Linhagem Brasileira | pt_BR |
| Subject in Portuguese | Sul do Brasil | pt_BR |
| Title | Multidrug-Resistant Mycobacterium tuberculosis of the Latin American Mediterranean Lineage, Wrongly Identified as Mycobacterium pinnipedii (Spoligotype International Type 863 [SIT863]), Causing Active Tuberculosis in South Brazil | pt_BR |
| Type | Article | |
| DOI | 10.1128/JCM.02012-15 | |
| Abstract | We recently detected the spoligotype patterns of strains of Mycobacterium pinnipedii, a species of the Mycobacterium tuberculosis complex, in sputum samples from nine cases with pulmonary tuberculosis residing in Porto Alegre, South Brazil. Because this species is rarely encountered in humans, we further characterized these nine isolates by additional genotyping techniques, including 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing, verification of the loci TbD1, RD9, pks15/1, RD(Rio), and fbpC, the insertion of IS6110 at a site specific to the M. tuberculosis Latin American Mediterranean (LAM) lineage, and whole-genome sequencing. The combined analysis of these markers revealed that the isolates are in fact M. tuberculosis and more specifically belong to the LAM genotype. Most of these isolates (n8) were shown to be multidrug resistant (MDR), which prompted us to perform partial sequencing of the rpoA, rpoB, rpoC, katG, and inhA genes. Seven isolates (77.8%) carried the S315T mutation in katG, and one of these (11%) also presented the C((-17)T single-nucleotide polymorphism (SNP) in inhA. Interestingly, six of the MDR isolates also presented an undescribed insertion of 12 nucleotides (CCA GAA CAA CCC) in codon 516 of rpoB. No putative compensatory mutation was found in either rpoA or rpoC. This is the first report of an M. tuberculosis LAM family strain with a convergent M. pinnipedii spoligotype. These spoligotypes are observed in genotype databases at a modest frequency, highlighting that care must be taken when identifying isolates in the M. tuberculosis complex on the basis of single genetic markers. | pt_BR |
| Affilliation | Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Luterana do Brasil. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande.Faculdade de Medicina. Nùcleo de Pesquisa em Biologia Médica. Rio Grande, RS, Brasil. | pt_BR |
| Affilliation | Universidade de Lisboa. Faculdade de Farmácia. Instituto de Investigação do Medicamento. Lisboa, Portugal. | pt_BR |
| Affilliation | Universidade de Lisboa. Faculdade de Farmácia. Instituto de Investigação do Medicamento. Lisboa, Portugal. | pt_BR |
| Affilliation | Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Unidade de Microbiologia Médica. Saúde Global e Medicina Tropical. Lisboa, Portugal. | pt_BR |
| Affilliation | London School of Hygiene & Tropical Medicine. Faculty of Infectious and Tropical Diseases. London, United Kingdom. | pt_BR |
| Affilliation | King Abdullah University of Science and Technology. Biological and Environmental Sciences and Engineering Division. Thuwal, Kingdom of Saudi Arabia. | pt_BR |
| Affilliation | London School of Hygiene & Tropical Medicine. Faculty of Infectious and Tropical Diseases. London, United Kingdom / London School of Hygiene & Tropical Medicine. Faculty of Epidemiology and Population Health. London, United Kingdom. | pt_BR |
| Affilliation | Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobactéries. Les Abymes, Guadeloupe, France. | pt_BR |
| Affilliation | Hospital Sanatório Partenon. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Fundação Estadual de Produção e Pesquisa em Saúde. Porto Alegre, RS, Brasil / Universidade Luterana do Brasil. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ. Brasil / Unit of Mycobacteriology at the Institute of Tropical Medicine. Antwerp, Belgium. | pt_BR |
| Subject | Tuberculosis | pt_BR |
| Subject | Mycobacterium tuberculosis | pt_BR |
| Subject | Multidrug-Resistant | pt_BR |
| Subject | Mycobacterium pinnipedii | pt_BR |
| Subject | Latin America | pt_BR |
| Subject | Mediterranean lineage | pt_BR |
| Subject | South Brazil | pt_BR |
| e-ISSN | 1098-660X | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
