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AutorNuovo, Allison J.
AutorGarofalo, Michela
AutorMikhail, Alexandria
AutorNicol, Alcina F.
AutorAndrade, Cecilia Vianna de
AutorNuovo, Gerard J.
Fecha de acceso2018-01-16T11:29:26Z
Fecha de disponibilización2018-01-16T11:29:26Z
Fecha de publicación2013
ReferenciaNUOVO, Allison J. et al. The Effect of Aging of Formalin-fixed Paraffin-embedded Tissues on the In Situ Hybridization and Immunohistochemistry Signals in Cervical Lesions. Diagn Mol Pathol., v. 00, n.00, 10p, 2013.pt_BR
ISSN1052-9551pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/23919
Idiomaengpt_BR
EditorLippincott, Williams & Wilkinspt_BR
Derechos de autorrestricted access
Palabras clave en Portuguésimuno-histoquímicapt_BR
Palabras clave en PortuguésFormaldeídopt_BR
Palabras clave en Portuguésparafinapt_BR
Palabras clave en PortuguésIdosospt_BR
TítuloThe Effect of Aging of Formalin-fixed Paraffin-embedded Tissues on the In Situ Hybridization and Immunohistochemistry Signals in Cervical Lesionspt_BR
Tipo del documentoArticle
Resumen en InglésFormalin-fixed, paraffin-embedded tissues are widely used in biomedical research but little is known about the effect of the age of the block or unstained slides on the in situ hybridization or immunohistochemistry signal. We compared the in situ-based and immunohistochemistry-based signals for cervical intraepithelial neoplasia samples that ranged from 0 to 15 years of age. There was a progressive and statistically significant decrease in the strength of the p16INK4a signal when comparing tissues prepared from recent unstained slides (0 to 1 y old, mean score of 92%) to those of intermediate age (5 to 7 y old, mean score of 49%) to old unstained slides (cut 13 to 15 y ago, mean score of 10%). Equivalent, progressive, and significant decreases in the intensity of the signals for microRNAs, CD45, and human papillomavirus DNA were seen in tissues stored on slides from 5 to 7 years and 13 to 15 years, respectively. However, the diminution of signal was much less, although still statistically significant, if the sections from the 13- to 15-year-old paraffin blocks were prepared in 2012. The data likely does not represent degradation of the targets as extraction of several microRNA from the old blocks showed no detectable degradation, despite the markedly weakened in situ hybridization signal. It is concluded that in situ-based signal for DNA, microRNAs, and proteins in paraffin-embedded tissues are significantly reduced over time, especially when stored long term on glass slides which, in turn, can lead to a significant underestimation of the amount and presence of the nucleic acid or protein target.pt_BR
AfiliaciónThe Ohio State University Medical Center. Ohio, USA.pt_BR
AfiliaciónThe Ohio State University. The Comprehensive Cancer Center. Ohio, USA.pt_BR
AfiliaciónThe Ohio State University. Columbus, USA.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Fernandes Figueira. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaciónThe Ohio State University. The Comprehensive Cancer Center. Ohio, USA / Phylogeny Inc.. Powell, OH, USA.pt_BR
Palavras clave en Inglêsimmunohistochemistrypt_BR
Palavras clave en Inglêsformalinpt_BR
Palavras clave en Inglêsparaffinpt_BR
Palavras clave en Inglêsagedpt_BR
e-ISSN1533-4066
Fecha de embargo2030-01-01


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