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| Autor | Andrade, Dafne Carvalho | |
| Autor | Borges, Igor Carmo | |
| Autor | Ekström, N. | |
| Autor | Jartti, T. | |
| Autor | Puhakka, T. | |
| Autor | Barral, Aldina Maria Prado | |
| Autor | Kayhty, H. | |
| Autor | Ruuskanen, O. | |
| Autor | Carvalho, Cristiana Maria Costa Nascimento de | |
| Fecha de acceso | 2018-02-05T14:06:18Z | |
| Fecha de disponibilización | 2018-02-05T14:06:18Z | |
| Fecha de publicación | 2018 | |
| Referencia | ANDRADE, D. C. et al. Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings. European Journal of Clinical Microbiology and Infectious Diseases, out. 2017. | pt_BR |
| ISSN | 0934-9723 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/24709 | |
| Promoción | Bahia State Agency for Research Funding (FAPESB), Brazil; Brazilian Council for Scientific and Technological Development (CNPq), Brazil; Turku University Hospital Research Foundation, Finland; Rauno and Anne Puolimatka Foundation, Finland; Sohlberg Foundation, Finland. | pt_BR |
| Idioma | eng | pt_BR |
| Editor | Springer Verlag | pt_BR |
| Derechos de autor | open access | pt_BR |
| Palabras clave en Portugués | Streptococcus pneumoniae | pt_BR |
| Palabras clave en Portugués | Infecções pneumococicas | pt_BR |
| Palabras clave en Portugués | Proteínas | pt_BR |
| Palabras clave en Portugués | Crianças | pt_BR |
| Título | Determination of avidity of IgG against protein antigens from Streptococcus pneumoniae: assay development and preliminary application in clinical settings | pt_BR |
| Tipo del documento | Article | pt_BR |
| DOI | 10.1007/s10096-017-3103-8 | |
| Resumen en Inglés | The measurement of antibody levels is a common test for the diagnosis of Streptococcus pneumoniae infection in research. However, the quality of antibody response, reflected by avidity, has not been adequately evaluated. We aimed to evaluate the role of avidity of IgG against eight pneumococcal proteins in etiologic diagnosis. Eight pneumococcal proteins (Ply, CbpA, PspA1 and 2, PcpA, PhtD, StkP-C, and PcsB-N) were used to develop a multiplex bead-based avidity immunoassay. The assay was tested for effects of the chaotropic agent, multiplexing, and repeatability. The developed assay was applied to paired samples from children with or without pneumococcal disease (n = 38 for each group), determined by either serology, polymerase chain reaction (PCR), or blood culture. We found a good correlation between singleplex and multiplex assays, with r ≥ 0.94.The assay was reproducible, with mean inter-assay variation ≤ 9% and intra-assay variation < 6%. Children with pneumococcal disease had lower median avidity indexes in the acute phase of disease for PspA1 and 2 (p = 0.042), PcpA (p = 0.002), PhtD (p = 0.014), and StkP-C (p < 0.001). When the use of IgG avidity as a diagnostic tool for pneumococcal infection was evaluated, the highest discriminative power was found for StkP-C, followed by PcpA (area under the curve [95% confidence interval, CI]: 0.868 [0.759-0.977] and 0.743 [0.607-879], respectively). The developed assay was robust and had no deleterious influence from multiplexing. Children with pneumococcal disease had lower median avidity against five pneumococcal proteins in the acute phase of disease compared to children without disease. | pt_BR |
| Afiliación | Federal University of Bahia. School of Medicine. Postgraduate Programme in Health Sciences. Salvador, BA, Brazil | pt_BR |
| Afiliación | Federal University of Bahia. School of Medicine. Postgraduate Programme in Health Sciences. Salvador, BA, Brazil | pt_BR |
| Afiliación | National Institute for Health and Welfare. Helsinki, Finland | pt_BR |
| Afiliación | University of Turku and Turku University Hospital. Department of Paediatrics. Turku, Finland | pt_BR |
| Afiliación | University of Turku and Turku University Hospital. Department of Paediatrics. Turku, Finland / Satakunta Central Hospital. Department of Otorhinolaryngology. Pori, Finland | pt_BR |
| Afiliación | Federal University of Bahia. School of Medicine. Pathology Department and Postgraduate Programme in Health Sciences. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Afiliación | University of Turku and Turku University Hospital. Department of Paediatrics. Turku, Finland | pt_BR |
| Afiliación | University of Turku and Turku University Hospital. Department of Paediatrics. Turku, Finland / Satakunta Central Hospital. Department of Otorhinolaryngology. Pori, Finland | pt_BR |
| Afiliación | Federal University of Bahia. School of Medicine. Pathology Department and Postgraduate Programme in Health Sciences. Salvador, BA, Brazil | pt_BR |
| Palavras clave en Inglês | Streptococcus pneumoniae | pt_BR |
| Palavras clave en Inglês | Infection | pt_BR |
| Palavras clave en Inglês | Protein | pt_BR |
| Palavras clave en Inglês | Children | pt_BR |
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