| Author | Assis, Luciana Moura de | |
| Author | Sousa, J. R | |
| Author | Pinto, N. F. S | |
| Author | Silva, A. A | |
| Author | Vaz, Antonio Fernando de Melo | |
| Author | Andrade, Paulo Paes | |
| Author | Carvalho, Edgar Marcelino de | |
| Author | Melo, Marcia Almeida de | |
| Access date | 2018-02-20T14:18:03Z | |
| Available date | 2018-02-20T14:18:03Z | |
| Document date | 2014 | |
| Citation | ASSIS, L. M. et al. B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis. Parasite Immunology, v. 36, p. 313–323, 2014. | pt_BR |
| ISSN | 0141-9838 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/24882 | |
| Description | CARVALHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
| Sponsorship | National Institute of Science and Technology of Tropical Diseases (Instituto Nacional de
Ci^encia e Tecnologia de Doenc as Tropicais – INCT-DT), MCT/CNPq, | pt_BR |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Bioinformática | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | Kala-azar | pt_BR |
| Subject in Portuguese | Biologia sintética | pt_BR |
| Subject in Portuguese | Vacina | pt_BR |
| Title | B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1111/pim.12111 | |
| Abstract | Serodiagnosis of visceral leishmaniasis is often hindered by cross-reactions to other parasitic diseases. Identifying specific B-cell epitopes in proteins is therefore important for immunodiagnostics, as well as for disease control by vaccines. This study aimed to identify linear and conformational B-cell epitopes and to evaluate the secondary structure of antigen proteins in Leishmania infantum using in silico analysis. Linear epitopes were predicted using the Immune Epitope Database and Analysis Resource (IEDB), BepiPred and BcePred programs. The conformational B-cell epitopes were identified using the CBTOPE server. The combination of the predictions using IEDB, BepiPred and BcePred generated 148 linear epitopes from the calpain-like cysteine peptidase (CP), thiol-dependent reductase 1 (TDR1) and HSP70 proteins. In total, 164 conformational epitopes were predicted, mostly located in the linear epitope region. The predicted epitopes are located in α helix and random coil regions in the thiol-dependent reductase 1 and HSP70 proteins. New linear and conformational B-cell epitopes of L. infantum proteins were identified in silico, and the prediction using various programs ensures greater accuracy of the results, as suggested by confirmation of previously identified HSP70 epitopes. | pt_BR |
| Affilliation | Federal University of Bahia. University Hospital Complex Professor Edgard Santos. Program in Medicine and Health. Salvador, BA, Brazil / Federal University of Campina Grande. Academic Nursing Unit. Cajazeiras, CG, Brazil | pt_BR |
| Affilliation | Faculdade Santa Maria. Santa Maria College. Cajazeiras, CG, Brazil | pt_BR |
| Affilliation | Federal University of Campina Grande. Veterinary Medicine Graduate. Program in Veterinary Medicine Academic Unit. Patos, CG, Brasil | pt_BR |
| Affilliation | Federal University of Campina Grande. Academic Unit of Exact and Natural Sciences. Cajazeiras, CG, Brazil | pt_BR |
| Affilliation | Federal University of Campina Grande. Academic Unit Veterinary Medicine. Patos, CG, Brazil | pt_BR |
| Affilliation | Federal University of Pernambuco. Department of Genetics. Recife, PE, Brazil | pt_BR |
| Affilliation | Federal University of Bahia. Professor Edgard Santos University Hospital Complex. Graduate Program in Medicine and Health, Immunology Service. Salvador, BA, Brazil / National Institute of Science and Technology of Tropical Diseases. Salvador, BA, Brazil | pt_BR |
| Affilliation | Federal University of Campina Grande. Academic Unit Veterinary Medicine. Patos, CG, Brazil | pt_BR |
| Subject | Bioinformatics | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Kala-azar | pt_BR |
| Subject | Synthetic biology | pt_BR |
| Subject | Vaccine | pt_BR |
