Use este identificador para citar ou linkar para este item:
IN VITROIMMUNOMODULATORY ACTIVITY OF A TRANSITION-STATE ANALOG INHIBITOR OF HUMAN PURINE NUCLEOSIDE PHOSPHORYLASE IN CUTANEOUS LEISHMANIASIS
Purina-nucleósideo fosforilase
Linfócitos
Leishmania
Citometria de fluxo
Humanos
Purine Nucleoside Phosphorylase
Lymphocyte
Leishmania
Flow cytometry
Humans
Autor(es)
Afiliação
Resumo em Inglês
Cutaneous leishmaniasis (CL) is the most common clinical form of American tegumentary leishmaniasis caused byLeishmania(Viannia)braziliensis. CL is associated with a strong Th1 immune response. This exacerbated inflammatory response is correlated with severity of disease and delays the healing time of the ulcer. The fourth-generation immucillin derivative (DI4G), a potent inhibitor of purine nucleoside phosphorylase, has been proposed as a promising agent in the treatment of diseases associated with T cell activation. Herein, we evaluated thein vitroimmunomodulatory activity of DI4G in cells of patients presenting with CL. Peripheral blood mononuclear cells (PBMC) from CL patients were stimulated with soluble leishmania antigen (SLA), in the presence or absence of DI4G, and IFN-γ, TNF, CXCL9, and CXCL10 levels were determined by ELISA. Lymphocyte proliferation in the presence or absence of DI4G was also evaluated, using flow cytometry. DI4G was able to decrease (p< 0.05) IFN-γproduction but did not change the TNF, CXCL9, and CXCL10 levels. DI4G decreased (p< 0.05) the lymphoproliferative response mediated by CD8+T cells, but not that by CD4+T cells. DI4G is able to attenuate the exaggerated immune response in CL, exhibiting immunomodulatory activity in IFN-γproduction and in CD8+T cell proliferation.
Palavras-chave
Leishmaniose CutâneaPurina-nucleósideo fosforilase
Linfócitos
Leishmania
Citometria de fluxo
Humanos
Palavras-chave em inglês
Cutaneous LeishmaniasisPurine Nucleoside Phosphorylase
Lymphocyte
Leishmania
Flow cytometry
Humans
Compartilhar