Author | Lonien, Sandra C. H. | |
Author | Malvezi, Aparecida Donizette | |
Author | Suzukawa, Helena T. | |
Author | Yamauchi, Lucy Megumi | |
Author | Yamada-Ogatta, Sueli Fumie | |
Author | Rizzo, Luiz V. | |
Author | Bordignon, Juliano | |
Author | Pinge-Filho, Phileno | |
Access date | 2018-02-23T19:51:52Z | |
Available date | 2018-02-23T19:51:52Z | |
Document date | 2017 | |
Citation | LONIEN, Sandra C. H. et al. Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway. Frontiers in Microbiology, v. 8, n. 2020, p. 1-11, 2017. | pt_BR |
ISSN | 1664-302X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/24927 | |
Language | por | pt_BR |
Publisher | Frontiers Media | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Title | Response to Trypanosoma cruzi by human blood cells enriched with Dentritic Cells is controlled by Cyclooxygenase-2 Pathway | pt_BR |
Type | Article | pt_BR |
DOI | 10.3389/fmicb.2017.02020 | pt_BR |
Abstract | Chagas disease (Cd) or American human trypanosomiasis is caused by Trypanosoma cruzi and affects ~7 million people, mostly in Latin America. The infective trypomastigote forms of the parasite can invade several human blood cell populations, including monocytes and dendritic cells (DC). Although these cells display a wide functional diversity, their interactions with T. cruzi via cyclooxygenase (COX) and cyclic adenosine monophosphate (cAMP) dependent pathways have not been analyzed. To exploiting this mechanism, DC-enriched peripheral human blood mononuclear cell populations (DC-PBMC) were used as our model. Our results showed that the treatment of these cell populations with celecoxib (CEL), a cyclooxygenase-2 selective inhibitor or SQ 22,536, an adenilate cyclase inhibitor, significantly caused marked inhibition of T. cruzi infection. In contrast, aspirin (ASA, a non-selective COX-1 and COX-2 inhibitor) treatment did not inhibit the infection of the cells by the parasite and was independent of nitric oxide (NO) production. The expression of co-stimulatory molecules CD80 and CD86 were similar on cells treated or not with both COX-inhibitors. The infection stimulated the release of TNF-α, IL-1β, IL-6, IL-8, and IL-10 production by infected cells. Treatment with ASA or CEL did not affect TNF-α, IL-6, IL-8, IL-10, and NO production by infected cells, but increased IL-1β production by them. Our results suggest a key role of COX-2 and cAMP pathways in T. cruzi invasion process of human blood cells and these pathways may represent targets of new therapeutic options for Cd. | pt_BR |
Affilliation | Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil. | pt_BR |
Affilliation | Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil. | pt_BR |
Affilliation | Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil. | pt_BR |
Affilliation | Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Biologia Molecular de Microrganismos, Londrina, PR, Brasil. | pt_BR |
Affilliation | Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Laboratório de Biologia Molecular de Microrganismos, Londrina, PR, Brasil. | pt_BR |
Affilliation | Hospital Israelita Albert Einstein, São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunologia. Londrina, PR, Brasil. | pt_BR |
Subject | Dendritic Cells | pt_BR |
Subject | Aspirin | pt_BR |
Subject | Celecoxib | pt_BR |
Subject in Spanish | Células Dendríticas | pt_BR |
Subject in Spanish | Aspirin | pt_BR |
Subject in Spanish | Celecoxib | pt_BR |
DeCS | Células Dendríticas | pt_BR |
DeCS | Aspirina | pt_BR |
DeCS | Celecoxib | pt_BR |
e-ISSN | 10.3389/fmicb.2017.02020 | |