| Author | Martiniano, Nicole O. Moura | |
| Author | Ferreira, Erik Machado | |
| Author | Gazêta, Gilberto S. | |
| Author | Soares, Carlos Augusto Gomes | |
| Access date | 2018-02-27T16:06:16Z | |
| Available date | 2018-02-27T16:06:16Z | |
| Document date | 2017 | |
| Citation | MARTINIANO, Nicole O. Moura; et al. Relative transcription of autophagy-related genes in Amblyomma sculptum and Rhipicephalus microplus ticks. Exp Appl Acarol., v. 73, p.401–428, 2017. | pt_BR |
| ISSN | 0168-8162 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/24973 | |
| Language | eng | pt_BR |
| Publisher | Springer Verlag (Germany) | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Amblyomma sculptum | pt_BR |
| Subject in Portuguese | Rhipicephalus microplus | pt_BR |
| Subject in Portuguese | Ixodidae Heteroxenous / monoxenous | pt_BR |
| Subject in Portuguese | Genes relacionados à autofagia | pt_BR |
| Title | Relative transcription of autophagy-related genes in Amblyomma sculptum and Rhipicephalus microplus ticks | pt_BR |
| Type | Article | |
| DOI | 10.1007/s10493-017-0193-z | |
| Abstract | Ticks endure stressful off-host periods and perform as vectors of a diversity of infectious agents, thus engaging pathways that expectedly demand for autophagy. Little is known of ticks' autophagy, a conserved eukaryotic machinery assisting in homeostasis processes that also participates in tissue-dependent metabolic functions. Here, the autophagy-related ATG4 (autophagin-1), ATG6 (beclin-1) and ATG8 (LC3) mRNAs from the human diseases vector Amblyomma sculptum and the cattle-tick Rhipicephalus microplus were identified. Comparative qPCR quantifications evidenced different transcriptional status for the ATG genes in the salivary glands (SG), ovaries and intestines of actively feeding ticks. These ATGs had increased relative transcription under nutrient-deprivation, as determined by validation tests with R. microplus embryo-derivative cells BME26 and A. sculptum SG explants incubations in HBSS. Starvation lead to 4-31.8× and ~ 60-500× increments on the ATGs mRNA loads in BME26 and A. sculptum SG explants, respectively. PI3K inhibitor 3MA treatment also affected ATGs expression in BME26. Some ATGs were more transcribed in the SGs than in the ovaries of cattle-ticks. Amblyomma sculptum/R. microplus interspecific comparisons showed that ATG4 and ATG6 were 0.18× less expressed in A. sculptum SGs, but ~ 10-100× more expressed in their ovaries when compared to R. microplus organs. ATG4 and ATG8a transcript loads were ~ 120× and ~ 40× higher, respectively, in A. sculptum intestines when compared to cattle-ticks of similar weight category. ATGs expression in A. sculptum intestines increased with tick weight, indicating Atgs contribution to intracellular blood digestion. Possible roles of the autophagy machinery and their organ-specific expression profile on vector biology are discussed. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Genética Molecular de Eucariontes e Simbiontes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Genética Molecular de Eucariontes e Simbiontes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores de Riquetsioses. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Genética Molecular de Eucariontes e Simbiontes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Autophagy related genes | pt_BR |
| Subject | Heteroxenous/monoxenous Ixodidae | pt_BR |
| Subject | Amblyomma cajennense sensu lato | pt_BR |
| Subject | Amblyomma sculptum | pt_BR |
| Subject | Rhipicephalus microplus | pt_BR |
| Subject | ATG4/ATG6/ ATG8 transcripts | pt_BR |
| Subject | BME26 tick cells | pt_BR |
| e-ISSN | 1572-9702 | |
| Embargo date | 2030-01-01 | |
