| Author | Cardoso, Thiago Marconi | |
| Author | Machado, Álvaro | |
| Author | Costa, Diego Luiz | |
| Author | Carvalho, Lucas Pedreira de | |
| Author | Queiroz, Adriano | |
| Author | Machado, Paulo | |
| Author | Scott, Phillip | |
| Author | Carvalho Filho, Edgar Marcelino | |
| Author | Bacellar, Olívia | |
| Access date | 2018-02-27T16:57:16Z | |
| Available date | 2018-02-27T16:57:16Z | |
| Document date | 2015 | |
| Citation | CARDOSO, T. M. et al. Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection. Infection and Immunity, v. 83, n. 3, p. 898-906, 2015. | pt_BR |
| ISSN | 0019-9567 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/24983 | |
| Description | CARVALHO FILHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
| Sponsorship | National Institutes of Health (NIH) grant AI30639-20. | pt_BR |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmania braziliensis | pt_BR |
| Subject in Portuguese | Leishmaniose Cutânea | pt_BR |
| Subject in Portuguese | Monócitos | pt_BR |
| Subject in Portuguese | Interferon gama | pt_BR |
| Subject in Portuguese | Fator de necrose tumoral alfa | pt_BR |
| Title | Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1128/IAI.02404-14 | |
| Abstract | Cutaneous leishmaniasis (CL) caused by Leishmania braziliensis is characterized by a strong Th1 response that leads to skin lesion development. In areas where L. braziliensis transmission is endemic, up to 15% of healthy subjects have tested positive for delayed-type hypersensitivity to soluble leishmania antigen (SLA) and are considered to have subclinical (SC) infection. SC subjects produce less gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) than do CL patients, but they are able to control the infection. The aim of this study was to characterized the role of CD8(+) T cells in SC infection and in CL. Peripheral blood mononuclear cells (PBMC) were stimulated with SLA to determine the frequencies of CD4(+) IFN-γ(+) and CD8(+) IFN-γ(+) T cells. Monocytes from PBMC were infected with L. braziliensis and cocultured with CD8(+) T cells, and the frequencies of infected monocytes and levels of cytotoxicity markers, target cell apoptosis, and granzyme B were determined. The frequency of CD8(+) IFN-γ(+) cells after SLA stimulation was higher for SC individuals than for CL patients. The frequency of infected monocytes in SC cells was lower than that in CL cells. CL CD8(+) T cells induced more apoptosis of infected monocytes than did SC CD8(+) T cells. Granzyme B production in CD8(+) T cells was higher in CL than in SC cells. While the use of a granzyme B inhibitor decreased the number of apoptotic cells in the CL group, the use of z-VAD-FMK had no effect on the frequency of these cells. These results suggest that CL CD8(+) T cells are more cytotoxic and may be involved in pathology. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, Brazil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, Pennsylvania, USA | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, Brasil | pt_BR |
| Subject | Leishmania braziliensis | pt_BR |
| Subject | Leishmaniasis, Cutaneous | pt_BR |
| Subject | Monocytes | pt_BR |
| Subject | Interferon-gamma | pt_BR |
| Subject | Tumor Necrosis Factor-alpha | pt_BR |
