Author | Costa, Diego Luis | |
Author | Cardoso, Thiago Marconi de Souza | |
Author | Queiroz, Adriano | |
Author | Milanezi, Cristiane Maria | |
Author | Bacellar, Maria Olívia Amado Ramos | |
Author | Carvalho Filho, Edgar Marcelino | |
Author | Silva, João Santana da | |
Access date | 2018-03-05T18:07:33Z | |
Available date | 2018-03-05T18:07:33Z | |
Document date | 2015 | |
Citation | COSTA, D. L. et al. Tr-1-like CD4+CD25-CD127-/lowFOXP3- cells are the main source of interleukin 10 in patients with cutaneous leishmaniasis due to Leishmania braziliensis. The Journal of Infectious Diseases, v. 211, p. 708–718, 2015. | pt_BR |
ISSN | 0022-1899 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/25061 | |
Description | Carvalho Filho, Edgar Marcelino. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
Sponsorship | National Institutes of Health (grant AI30639-020) and the Fundação de Apoio à Pesquisa do Estado de São Paulo (grants 2008/05982-8 and 2007/53940-0). | pt_BR |
Language | eng | pt_BR |
Publisher | Oxford University Press | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | L. braziliensis | pt_BR |
Subject in Portuguese | leishmaniose tegumentar | pt_BR |
Subject in Portuguese | Células Tr-1 | pt_BR |
Subject in Portuguese | IL-10 | pt_BR |
Title | Tr-1-like CD4+CD25-CD127-/lowFOXP3- cells are the main source of interleukin 10 in patients with cutaneous leishmaniasis due to Leishmania braziliensis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1093/infdis/jiu406 | |
Abstract | CD4(+)CD25(+)FOXP3(+) regulatory T cells have long been shown to mediate susceptibility to Leishmania infection, mainly via interleukin 10 production. In this work, we showed that the main sources of interleukin 10 in peripheral blood mononuclear cells (PBMCs) from patients with cutaneous leishmaniasis due to Leishmania braziliensis are CD4(+)CD25(-)CD127(-/low)FOXP3(-) cells. Compared with uninfected controls, patients with CL had increased frequencies of circulating interleukin 10-producing CD4(+)CD25(-)CD127(-/low) cells, which efficiently suppressed tumor necrosis factor α production by the total PBMC population. Also, in CL lesions, interleukin 10 was mainly produced by CD4(+)CD25(-) cells, and interleukin 10 messenger RNA expression was associated with interleukin 27, interleukin 21, and interferon γ expression, rather than with FOXP3 or transforming growth factor β expressions. Active production of both interleukin 27 and interleukin 21, together with production of interferon γ and interleukin 10, was also detected in the lesions. Since these cytokines are associated with the differentiation and activity of Tr-1 cells, our results suggest that this cell population may play an important role in the immunomodulation of CL. Therefore, development of treatments that interfere with this pathway may lead to faster parasite elimination. | pt_BR |
Affilliation | University of São Paulo. Ribeirão Preto Medical School. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brazil | pt_BR |
Affilliation | Federal University of Bahia. University Hospital Professor Edgar Santos. Immunology Service. Salvador, BA, Brazil / National Institute of Science and Technology in Tropical Diseases. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. University Hospital Professor Edgar Santos. Immunology Service. Salvador, BA, Brazil / National Institute of Science and Technology in Tropical Diseases. Salvador, BA, Brasil | pt_BR |
Affilliation | University of São Paulo. Ribeirão Preto Medical School. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brazil | pt_BR |
Affilliation | Federal University of Bahia. University Hospital Professor Edgar Santos. Immunology Service. Salvador, BA, Brazil / National Institute of Science and Technology in Tropical Diseases. Salvador, BA, Brasil | pt_BR |
Affilliation | Federal University of Bahia. University Hospital Professor Edgar Santos. Immunology Service. Salvador, BA, Brazil / National Institute of Science and Technology in Tropical Diseases. Salvador, BA, Brasil | pt_BR |
Affilliation | University of São Paulo. Ribeirão Preto Medical School. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brazil | pt_BR |
Subject | L. braziliensis | pt_BR |
Subject | Tegumentary leishmaniasis | pt_BR |
Subject | Tr-1 cells | pt_BR |
Subject | IL-10 | pt_BR |