| Author | Gontijo, Raquel Carvalho | |
| Author | Moreira, Diana Raquel | |
| Author | Resende, Mariana | |
| Author | Silva, Matheus Fernandes Costa | |
| Author | Pascoal, Vanessa Peruhype Magalhães | |
| Author | Ribeiro, Cláudia Maria Franco | |
| Author | Ribeiro, Daniel Dias | |
| Author | Silvestre, Ricardo | |
| Author | Silva, Anabela Cordeiro da | |
| Author | Martins Filho, Olindo Assis | |
| Author | Carvalho, Andréa Teixeira de | |
| Access date | 2018-03-07T13:00:11Z | |
| Available date | 2018-03-07T13:00:11Z | |
| Document date | 2017 | |
| Citation | GONTIJO, Raquel Carvalho et al. Infection of hematopoietic stem cells by Leishmania infantum increases erythropoiesis and alters the phenotypic and functional profiles of progeny. Cell Immunol, pii: S0008-8749(17)30197-1, 2017 | pt_BR |
| ISSN | 0008-8749 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25097 | |
| Language | eng | pt_BR |
| Publisher | Elsevier B.V | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | citocinas | pt_BR |
| Subject in Portuguese | Leishmaniose | pt_BR |
| Title | Infection of hematopoietic stem cells by Leishmania infantum increases erythropoiesis and alters the phenotypic and functional profiles of progeny | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.cellimm.2017.10.016 | |
| Abstract | Immunosuppression is a well-established risk factor for Visceral Leishmaniasis. Post-immunosuppression leishmaniasis is characterized by an increase of parasite burden, hematopoietic disorders and unusual clinical manifestations. Although there are many reports on bone marrow findings in VL, less is known about the relationship between parasite dynamics in this organ and the function of either hematopoietic stem cells and progenitor cells themselves. In the present study, we tackle these issues using a new approach of infecting human stem cells derived from bone marrow with L. infantum. Using this strategy, we show that human hematopoietic stem cells (hHSC) are able to phagocytize L. infantum promastigotes and release modulatory and pro-inflammatory cytokines, mainly TNF-α. Our results demonstrated that L. infantum infection in vitro enhances hematopoiesis, favoring the development of erythrocitic lineage through a mechanism yet unknown. Moreover, we found that L. infantum infection alters the phenotypic profile of the hematopoietic progeny; modifying the surface markers expression of differentiated cells. Thus, our study represents a rare opportunity to monitor the in vitro differentiation of human stem cells experimentally infected by L. infantum to better understand the consequences of the infection on phenotypic and functional profile of the cell progeny. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisa em Biomarcadores. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Instituto de Biologia Molecular e Celular. Porto, Portugal/I3S - Instituto de Investigação e Inovação em Saúde. Porto, Portugal | pt_BR |
| Affilliation | Instituto de Biologia Molecular e Celular. Porto, Portugal/I3S - Instituto de Investigação e Inovação em Saúde. Porto, Portugal/ University of Minho. School of Medicine. Life and Health Sciences Research Institute. Braga, Portugal/ ICVS/3B's-PT Government Associate Laboratory. Braga, Portugal | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisa em Biomarcadores. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisa em Biomarcadores. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Clínica Hematológica. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Clínica Hematológica. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | University of Minho. School of Medicine. Life and Health Sciences Research Institute. Braga, Portugal/ ICVS/3B's-PT Government Associate Laboratory. Braga, Portugal | pt_BR |
| Affilliation | Instituto de Biologia Molecular e Celular. Porto, Portugal/I3S - Instituto de Investigação e Inovação em Saúde. Porto, Portugal/ Universidade do Porto. Faculdade de Farmácia. Porto, Portugal | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisa em Biomarcadores. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisa em Biomarcadores. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Cytokines | pt_BR |
| Subject | hematopoiesis | pt_BR |
| Subject | Hematopoietic progeny | pt_BR |
| Subject | Immunophenotyping | pt_BR |
| Subject | Leishmania | pt_BR |
| Subject | Stem cells | pt_BR |
| Embargo date | 2025-01-01 | |
