| Author | Nogueira, Paula Monalisa | |
| Author | Guimarães, Agna Cristina | |
| Author | Assis, Rafael Ramiro de | |
| Author | Sadlova, Jovana | |
| Author | Myskova, Jitka | |
| Author | Pruzinova, Katerina | |
| Author | Hlavackova, Jana | |
| Author | Turco, Salvatore J. | |
| Author | Torrecilhas, Ana Claudia Trocoli | |
| Author | Volf, Petr | |
| Author | Soares, Rodrigo Pedro Pinto | |
| Access date | 2018-03-07T16:36:12Z | |
| Available date | 2018-03-07T16:36:12Z | |
| Document date | 2017 | |
| Citation | NOGUEIRA, Paula Monalisa et al. Lipophosphoglycan polymorphisms do not affect Leishmania amazonensis development in the permissive vectors Lutzomyia migonei and Lutzomyia longipalpis. Parasit Vectors, v. 10, n. 1, p. 608, 2017 | pt_BR |
| ISSN | 1756-3305 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25140 | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmania amazonensis | pt_BR |
| Subject in Portuguese | Lutzomyia longipalpis | pt_BR |
| Subject in Portuguese | Lutzomyia migonei | pt_BR |
| Subject in Portuguese | Phlebotomus papatasi | pt_BR |
| Title | Lipophosphoglycan polymorphisms do not affect Leishmania amazonensis development in the permissive vectors Lutzomyia migonei and Lutzomyia longipalpis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1186/s13071-017-2568-8 | |
| Abstract | Background: Lipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes. Its species-specific polymorphisms are found mainly in the sugars that branch off the conserved Gal(β1,4)Man(α1)-PO4 backbone of repeat units. Leishmania amazonensis is one of the most important species causing human cutaneous leishmaniasis in the New World. Here, we describe LPG intraspecific polymorphisms in two Le. amazonensis reference strains and their role during the development in three sand fly species.
Results: Strains isolated from Lutzomyia flaviscutellata (PH8) and from a human patient (Josefa) displayed structural polymorphism in the LPG repeat units, possessing side chains with 1 and 2 β-glucose or 1 to 3 β-galactose, respectively. Both strains successfully infected permissive vectors Lutzomyia longipalpis and Lutzomyia migonei and could colonize their stomodeal valve and differentiate into metacyclic forms. Despite bearing terminal galactose residues on LPG, Josefa could not sustain infection in the restrictive vector Phlebotomus papatasi.
Conclusions: LPG polymorphisms did not affect the ability of Le. amazonensis to develop late-stage infections in permissive vectors. However, the non-establishment of infection in Ph. papatasi by Josefa strain suggested other LPG-independent factors in this restrictive vector. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG Brazil/Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG Brazil | pt_BR |
| Affilliation | Charles University. Faculty of Science. Department of Parasitology. Prague, Czech Republic | pt_BR |
| Affilliation | Charles University. Faculty of Science. Department of Parasitology. Prague, Czech Republic | pt_BR |
| Affilliation | Charles University. Faculty of Science. Department of Parasitology. Prague, Czech Republic | pt_BR |
| Affilliation | Charles University. Faculty of Science. Department of Parasitology. Prague, Czech Republic | pt_BR |
| Affilliation | University of Kentucky Medical Center. Department of Biochemistry. Lexington, KY USA | pt_BR |
| Affilliation | Universisade Federal de São Paulo. Departamento de Farmácia. Laboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários. São Paulo, SP Brazil | pt_BR |
| Affilliation | Charles University. Faculty of Science. Department of Parasitology. Prague, Czech Republic | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG Brazil | pt_BR |
| Subject | Leishmania amazonensis | pt_BR |
| Subject | Lipophosphoglycan | pt_BR |
| Subject | Lutzomyia longipalpis | pt_BR |
| Subject | Lutzomyia migonei | pt_BR |
| Subject | Phlebotomus papatasi | pt_BR |
| Subject | Vector-parasite interaction | pt_BR |
