| Autor | Belew, Ashton Trey | |
| Autor | Giusta, Caroline Junqueira | |
| Autor | Luiz, Gabriela Flavia Rodrigues | |
| Autor | Valente, Bruna Mattioly | |
| Autor | Oliveira, Antonio Edson Rocha | |
| Autor | Polidoro, Rafael Barbosa | |
| Autor | Zuccherato, Luciana Werneck | |
| Autor | Bartholomeu, Daniella Castanheira | |
| Autor | Schenkman, Sergio | |
| Autor | Gazzinelli, Ricardo Tostes | |
| Autor | Burleigh, Barbara A | |
| Autor | El-Sayed, Najib M. | |
| Autor | Teixeira, Santuza Maria Ribeiro | |
| Data de acesso | 2018-03-07T17:07:07Z | |
| Data de disponibilização | 2018-03-07T17:07:07Z | |
| Data do publicação | 2017 | |
| Citação | BELEW, Ashton Trey et al. Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection. PLoS Pathog., v. 13, n. 12, art. e1006767, 2017. | pt_BR |
| ISSN | 1553-7366 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25171 | |
| Idioma | eng | pt_BR |
| Editor | Public Library of Science | pt_BR |
| Direito Autoral | open access | pt_BR |
| Palavras-chave | Amastigotas | pt_BR |
| Palavras-chave | Trypanosoma cruzi | pt_BR |
| Título | Comparative transcriptome profiling of virulent and non-virulent Trypanosoma cruzi underlines the role of surface proteins during infection | pt_BR |
| Tipo do documento | Article | pt_BR |
| DOI | 10.1371/journal.ppat.1006767 | |
| Resumo em Inglês | Trypanosoma cruzi, the protozoan that causes Chagas disease, has a complex life cycle involving several morphologically and biochemically distinct stages that establish intricate interactions with various insect and mammalian hosts. It has also a heterogeneous population structure comprising strains with distinct properties such as virulence, sensitivity to drugs, antigenic profile and tissue tropism. We present a comparative transcriptome analysis of two cloned T. cruzi strains that display contrasting virulence phenotypes in animal models of infection: CL Brener is a virulent clone and CL-14 is a clone that is neither infective nor pathogenic in in vivo models of infection. Gene expression analysis of trypomastigotes and intracellular amastigotes harvested at 60 and 96 hours post-infection (hpi) of human fibroblasts revealed large differences that reflect the parasite’s adaptation to distinct environments during the infection of mammalian cells, including changes in energy sources, oxidative stress responses, cell cycle control and cell surface components. While extensive transcriptome remodeling was observed when trypomastigotes of both strains were compared to 60 hpi amastigotes, differences in gene expression were much less pronounced when 96 hpi amastigotes and trypomastigotes of CL Brener were compared. In contrast, the differentiation of the avirulent CL-14 from 96 hpi amastigotes to extracellular trypomastigotes was associated with considerable changes in gene expression, particularly in gene families encoding surface proteins such as trans-sialidases, mucins and the mucin associated surface proteins (MASPs). Thus, our comparative transcriptome analysis indicates that the avirulent phenotype of CL-14 may be due, at least in part, to a reduced or delayed expression of genes encoding surface proteins that are associated with the transition of amastigotes to trypomastigotes, an essential step in the establishment of the infection in the mammalian host. Confirming the role of members of the trans-sialidase family of surface proteins for parasite differentiation, transfected CL-14 constitutively expressing a trans-sialidase gene displayed faster kinetics of trypomastigote release in the supernatant of infected cells compared to wild type CL-14 | pt_BR |
| Afiliação | University of Maryland. College Park. Department of Cell Biology and Molecular Genetics and Center for Bioinformatics and Computational Biology. Maryland, United States of America | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil | pt_BR |
| Afiliação | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Universidade Federal de São Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Afiliação | Harvard T. H. Chan School of Public Health. Department of Immunology and Infectious Diseases. Boston, Massachusetts, United States of America | pt_BR |
| Afiliação | University of Maryland. College Park. Department of Cell Biology and Molecular Genetics and Center for Bioinformatics and Computational Biology. Maryland, United States of America | pt_BR |
| Afiliação | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Palavras-chave em inglês | Amastigotes | pt_BR |
| Palavras-chave em inglês | Trypomastigotes | pt_BR |
| Palavras-chave em inglês | Gene expression | pt_BR |
| Palavras-chave em inglês | Parasitic diseases | pt_BR |
| Palavras-chave em inglês | Trypanosoma cruzi | pt_BR |
| Palavras-chave em inglês | Cell differentiation | pt_BR |
| Palavras-chave em inglês | Epimastigotes | pt_BR |
| Palavras-chave em inglês | Comparative genomic | pt_BR |
