| Author | Prado, Isis C. | |
| Author | Souza, André L. A. | |
| Author | Provance, David W. | |
| Author | Cassella, Ricardo J. | |
| Author | De Simone, Salvatore Giovanni | |
| Access date | 2018-03-08T11:53:45Z | |
| Available date | 2018-03-08T11:53:45Z | |
| Document date | 2017 | |
| Citation | PRADO, Isis C. et al. Ultrasensitive and rapid immuno-detection of human IgE antitherapeutic horse sera using an electrochemical immunosensor. Analytical Biochemistry, v.338, p.13-19, 2017. | pt_BR |
| ISSN | 0003-2697 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25180 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Masson | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Eletroquímica | pt_BR |
| Subject in Portuguese | Imunossensor amperométrico | pt_BR |
| Subject in Portuguese | Antígeno específico de cavalo | pt_BR |
| Subject in Portuguese | Reação anafilática | pt_BR |
| Subject in Portuguese | Anti veneno | pt_BR |
| Subject in Portuguese | Imunoglobulina G | pt_BR |
| Title | Ultrasensitive and rapid immuno-detection of human IgE anti-therapeutic horse sera using an electrochemical immunosensor | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.ab.2017.09.008 | |
| Abstract | Antivenom allergy disease mediated by patient IgE is an important public health care concern. To improve detection of hypersensitive individuals prior to passive antibody therapy, an amperometric immunosensor was developed to detect reactive human IgE. Whole horse IgG3 (hoIgG3) was immobilized onto the surface of carbon or gold screen-printed electrodes through a cross-linking solution of glutaraldehyde on a chitosan film. Sera from persons with a known allergic response to hoIgG3 or non-allergic individuals was applied to the sensor. Bound human IgE (humIgE) was detected by an anti-humIgE antibody through a quantitative amperometric determination by tracking via the electrochemical reduction of the quinone generated from the hydroquinone with the application of a potential of 25 mV. The optimal immunosensor configuration detected reactive humIgE at a dilution of 1:1800 of the human sera that represent a detection limit of 0.5 pg/mL. Stability testing demonstrated that through 20 cycles of a scan, the specificity and performance remained robust. The new immunosensor successfully detected humIgE antibodies reactive against hoIgG3, which could allow the diagnosis of potential allergenic patients needing therapeutic antivenom preparations from a horse. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia de Gestão da Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Química. Departamento de Química Analítica. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Fármacos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto de Química. Departamento de Química Analítica. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Fármacos. Rio de Janeiro, RJ. Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil | pt_BR |
| Subject | Amperometric immunosensor | pt_BR |
| Subject | Electrochemistry | pt_BR |
| Subject | Horse specific antigen | pt_BR |
| Subject | Horse IgG3 | pt_BR |
| Subject | Human IgE specific assay | pt_BR |
| Subject | Anaphylactic reaction | pt_BR |
| Subject | Antivenom | pt_BR |
| e-ISSN | 1096-0309 | |
| Embargo date | 2030-01-01 | |
