| Author | Nuovo, Gerard J. | |
| Author | Wu, Xin | |
| Author | Volinia, Stefano | |
| Author | Yan, Fengting | |
| Author | di Leva, Gianpiero | |
| Author | Chin, Nena | |
| Author | Nicol, Alcina F. | |
| Author | Jiang, Jinmai | |
| Author | Otterson, Gregory | |
| Author | Schmittgen, Thomas D. | |
| Author | Croce, Carlo | |
| Access date | 2018-03-08T16:27:35Z | |
| Available date | 2018-03-08T16:27:35Z | |
| Document date | 2010 | |
| Citation | NUOVO, Gerard J. et al. Strong Inverse Correlation Between MicroRNA-125b and Human Papillomavirus DNA in Productive Infection. Diagn Mol Pathol, v.19, n.3, p.135-143, Sept. 2010. | pt_BR |
| ISSN | 1052-9551 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25199 | |
| Language | eng | pt_BR |
| Publisher | Lippincott, Williams & Wilkins | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Papilomavírus Humano | pt_BR |
| Subject in Portuguese | hibridização in situ | pt_BR |
| Subject in Portuguese | MicroRNAs | pt_BR |
| Title | Strong inverse correlation between microRNA-125b and human papillomavirus DNA in productive infection | pt_BR |
| Type | Article | |
| DOI | 10.1097/PDM.0b013e3181c4daaa | |
| Abstract | Infection by the human papillomavirus (HPV) is a cause of cervical intraepithelial neoplasia (CIN) and cancer. microRNA (miRNA) in situ analysis of the transformation zone epithelia, the site of initial cervical HPV infection, showed that miRNAs let-7c, -99a, 26a, and 125b were the most abundantly expressed. In situ testing of CIN 1 showed a dramatic reduction in miR-125b expression in the koilocytes, the cytologic marker of productive HPV infection. A marked reduction in miR-125b was likewise observed in the HPV-infected cells of the condyloma acuminatum, verruca vulgaris, and epidermodysplasia verruciformis. Reverse transcriptase in situ polymerase chain reaction (PCR) showed that the pre-miRNA 125b was present in the koilocyte, suggesting direct inactivation of the mature miRNA. HEK cells transfected with only the antimiR-125b showed perinuclear halos equivalent to HPV-infected koilocytes. NIH 3T3 cells transfected with the HPV 16 full-length genome and mimetic miR-125b showed a marked reduction in viral DNA and protein synthesis by quantitative PCR and in situ-based analyses, respectively (P=0.002). Alternatively, cotransfection with anti-miR-125b and HPV 16 markedly increased HPV DNA (P=0.002). Sequence analyses showed strong homology between L2 of different HPV genotypes and miR-125b. Transfection with HPV 16 L2 resulted in a marked reduction in miR-125b levels in the NIH 3T3 cells. HPV L2-induced inactivation of miR-125b is associated with the classic cytologic changes of the koilocyte, and the exogenous application of mimetic miR-125b markedly inhibits HPV DNA synthesis. | pt_BR |
| Affilliation | Ohio State University. Comprehensive Cancer Center. Heart and Lung Institute. Columbus, OH, USA. | pt_BR |
| Affilliation | Ohio State University Internal Medicine. Columbus, OH, USA. | pt_BR |
| Affilliation | Ohio State University. Comprehensive Cancer Center. Heart and Lung Institute. Columbus, OH, USA / Ohio State University. Molecular Virology, Immunology and Medical Genetics. Columbus, OH, USA. | pt_BR |
| Affilliation | Ohio State University. Comprehensive Cancer Center. Heart and Lung Institute. Columbus, OH, USA. | pt_BR |
| Affilliation | Ohio State University. Molecular Virology, Immunology and Medical Genetics. Columbus, OH, USA. | pt_BR |
| Affilliation | Ohio State University. Enzo Clinical Laboratory. Columbus, OH, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Ohio State University. The College of Pharmacy. Columbus, OH, USA. | pt_BR |
| Affilliation | Ohio State University Internal Medicine. Columbus, OH, USA. | pt_BR |
| Affilliation | Ohio State University. The College of Pharmacy. Columbus, OH, USA. | pt_BR |
| Affilliation | Ohio State University Internal Medicine. Columbus, OH, USA / Ohio State University. Molecular Virology, Immunology and Medical Genetics. Columbus, OH, USA. | pt_BR |
| Subject | human papillomavirus | pt_BR |
| Subject | microRNA | pt_BR |
| Subject | in situ hybridization | pt_BR |
| Subject | L2 | pt_BR |
| e-ISSN | 1533-4066 | |
| Embargo date | 2030-01-01 | |
