| Author | Mello, Francisco C. A. | |
| Author | Martel, Nora | |
| Author | Gomes, Selma A. | |
| Author | Araujo, Natalia M. | |
| Access date | 2018-03-15T16:13:19Z | |
| Available date | 2018-03-15T16:13:19Z | |
| Document date | 2011 | |
| Citation | MELLO, Francisco C. A. et al. Expression of Hepatitis B Virus Surface Antigen Containing Y100C Variant Frequently Detected in Occult HBV Infection. Hepatitis Research and Treatment, Volume 2011, Article ID 695859, 4p, 2011. | pt_BR |
| ISSN | 2090-1364 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25320 | |
| Language | eng | pt_BR |
| Publisher | Hindawi Publishing Corporation | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Vírus da Hepatite B | pt_BR |
| Subject in Portuguese | Saúde Pública | pt_BR |
| Subject in Portuguese | Y100C | pt_BR |
| Subject in Portuguese | Antígeno | pt_BR |
| Title | Expression of Hepatitis B Virus Surface Antigen Containing Y100C Variant Frequently Detected in Occult HBV Infection | pt_BR |
| Type | Article | |
| DOI | 10.1155/2011/695859 | |
| Abstract | Small hepatitis B virus surface protein (S-HBsAg) variant Y100C has been associated with HBsAg-negative phenotype. To
determine whether Y100C substitution yields impaired HBsAg or small amounts of HBsAg that may reduce HBsAg detection
by commercial anti-HBsAg antibodies, two eukaryotic expression plasmids, one containing a wild-type S and the other an S
gene from a Y100C variant, were constructed and their levels of HBsAg compared by ELISA after transfection of HuH7 cells.
Unexpectedly, the extracellular HBsAg levels detected with Y100C plasmid were higher than those observed with the wild-type
plasmid, but without statistical significance. We concluded that the Y100C substitution alone did not play a role in reducing
HBsAg amounts or HBsAg affinity by commercial ELISA assay. Further studies on in vitro replication fitness with the complete
genome of HBV isolates displaying or not Y100C substitution may elucidate whether this mutation affects HBV replication and
consequently HBsAg production. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | INSERM. Lyon, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Hepatitis B Virus | pt_BR |
| Subject | PublicHealth | pt_BR |
| Subject | Y100C | pt_BR |
| Subject | antigen | pt_BR |
| Subject | Occult HBV Infection | pt_BR |
| e-ISSN | 2090-1372 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
