| Author | Rose, Tatiana L. | |
| Author | Miagostovich, Marize P. | |
| Author | Leite, José Paulo G. | |
| Access date | 2018-03-20T12:00:25Z | |
| Available date | 2018-03-20T12:00:25Z | |
| Document date | 2010 | |
| Citation | ROSE, Tatiana L.; MIAGOSTOVICH, Marize P.; LEITE, José Paulo G. Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain. Mem Inst Oswaldo Cruz, v.105, n.8, p.1068-1072, Dec. 2010. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25409 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Estirpe da vacina Rotarix® | pt_BR |
| Subject in Portuguese | restriction endonuclease | pt_BR |
| Subject in Portuguese | Rotavirus A | pt_BR |
| Subject in Portuguese | Infecções por Rotavirus | pt_BR |
| Title | Rotavirus A genotype G1P[8]: a novel method to distinguish wild-type strains from the Rotarix® vaccine strain | pt_BR |
| Type | Article | pt_BR |
| Abstract | Rotaviruses are important enteric pathogens for humans and animals. Group A rotaviruses (RV-A) are the
most common agents of severe gastroenteritis in infants and young children and vaccination is the most effective
method to reduce RV-A-associated diseases. G1P[8], the most prevalent RV-A genotype worldwide, is included
in the RV-A vaccine Rotarix®. The discrimination between wild-type G1P[8] and vaccine G1P[8] strains is an
important topic in the study of RV-A epidemiology to manage outbreaks and to define control measures for vaccinated
children. In this study, we developed a novel method to segregate the wild-type and vaccine strains using
restriction endonucleases. The dsRNA from the Rotarix® vaccine was sequenced and the NSP3 gene was selected
as the target gene. The vaccine strain has a restriction pattern that is different than that of wild-type RV-A G1P[8]
isolates after digestion with the restriction endonuclease BspHI. This pattern could be used as a marker for the
differentiation of wild-type G1P[8] strains from the vaccine strain. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | Rotarix® vaccine strain | pt_BR |
| Subject | G1P[8] wild-type strain | pt_BR |
| Subject | rotavirus A strain differentiation | pt_BR |
| Subject | restriction endonuclease | pt_BR |
| e-ISSN | 1678-8060 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
