| Author | Luiz, Lysa Nepomuceno | |
| Author | Leite, José Paulo Gagliardi | |
| Author | Yokosawa, Jonny | |
| Author | Carneiro, Bruno M. | |
| Author | Pereira Filho, Edson | |
| Author | Oliveira, Thelma Fátima de Mattos | |
| Author | Freitas, Guilherme Ramos Oliveira e | |
| Author | Costa, Lourenço Faria | |
| Author | Paula, Nayhanne Tizzo de | |
| Author | Silveira, Hélio Lopes da | |
| Author | Nepomuceno, Júlio César | |
| Author | Queiróz, Divina Aparecida Oliveira | |
| Access date | 2018-03-20T13:43:29Z | |
| Available date | 2018-03-20T13:43:29Z | |
| Document date | 2010 | |
| Citation | LUIZ, Lysa Nepomuceno; et al. Molecular characterization of adenoviruses from children presenting with acute respiratory disease in Uberlândia, Minas Gerais, Brazil, and detection of an isolate genetically related to feline adenovirus. Mem Inst Oswaldo Cruz, v.105, n.5, p.712-716, Aug. 2010. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25417 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Adenovirus | pt_BR |
| Subject in Portuguese | Crianças | pt_BR |
| Subject in Portuguese | doença respiratória aguda | pt_BR |
| Title | Molecular characterization of adenoviruses from children presenting with acute respiratory disease in Uberlândia, Minas Gerais, Brazil, and detection of an isolate genetically related to feline adenovirus | pt_BR |
| Type | Article | |
| Abstract | Human adenoviruses (HAdV) are a major cause of acute respiratory diseases (ARD), gastroenteritis, conjunctivitis and urinary infections. Between November 2000-April 2007, a total of 468 nasopharyngeal aspirate samples were collected from children with ARD at the Clinics Hospital of Uberlândia. These samples were tested by immunofluorescence assay (IFA) and 3% (14/468) tested positive for the presence of HAdV. By performing polymerase chain reaction (PCR) to detect HAdV DNA in samples that tested negative or inconclusive for all viruses identifiable by IFA (respiratory syncytial virus, parainfluenza viruses 1, 2 and 3, influenza viruses A and B and HAdV), as well as negative for rhinoviruses by reverse transcription-PCR, additional 19 cases were detected, for a total of 33 (7.1%) HAdV-positive samples. Nucleotide sequences of 13 HAdV samples were analyzed, revealing that they belonged to species B, C and E. Further analyses showed that species C (HAdV-2) was the most prevalent among the sequenced samples. To our knowledge, this is the first report describing the presence of HAdV-4 in Brazil. We also detected an isolate that was 100% identical to a part of the feline adenovirus hexon gene sequence. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Centers for Disease Control and Prevention. Division of Viral Hepatitis. Atlanta, GA, USA. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Faculdade de Medicina. Uberlândia, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Laboratório de Citogenética e Mutagênese. Uberlândia, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Virologia. Uberlândia, MG, Brasil. | pt_BR |
| Subject | adenovirus | pt_BR |
| Subject | acute respiratory disease | pt_BR |
| Subject | children | pt_BR |
| e-ISSN | 1678-8060 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
