| Author | Sousa, Antonio Ferreira Mendes | |
| Author | Vale, Vladimir Fazito | |
| Author | Queiroz, Daniel Costa | |
| Author | Pereira Filho, Adalberto Alves | |
| Author | Silva, Naylene Carvalho Sales da | |
| Author | Koerich, Leonardo Barbosa | |
| Author | Moreira, Luciano Andrade | |
| Author | Pereira, Marcos Horácio | |
| Author | Sant’Anna, Maurício Roberto | |
| Author | Araújo, Ricardo Nascimento | |
| Author | Andersen, John | |
| Author | Valenzuela, Jesus Gilberto | |
| Author | Gontijo, Nelder Figueiredo | |
| Access date | 2018-03-21T13:58:53Z | |
| Available date | 2018-03-21T13:58:53Z | |
| Document date | 2018 | |
| Citation | SOUSA, Antonio Ferreira Mendes et al. Inhibition of the complement system by saliva of Anopheles (Nyssorhynchus) aquasalis. Insect Biochemistry and Molecular Biology, v. 92, p. 12-20, 2018. | pt_BR |
| ISSN | 0965-1748 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25440 | |
| Language | eng | pt_BR |
| Publisher | Pergamon Press | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Anopheles aquasalis | pt_BR |
| Subject in Portuguese | sistema complementar | pt_BR |
| Title | Inhibition of the complement system by saliva of Anopheles (Nyssorhynchus) aquasalis | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.ibmb.2017.11.004 | |
| Abstract | Anopheline mosquitoes are vectors of malaria parasites. Their saliva contains anti-hemostatic and immune-modulator molecules that favor blood feeding and parasite transmission. In this study, we describe the inhibition of the alternative pathway of the complement system (AP) by Anopheles aquasalis salivary gland extracts (SGE). According to our results, the inhibitor present in SGE acts on the initial step of the AP blocking deposition of C3b on the activation surfaces. Properdin, which is a positive regulatory molecule of the AP, binds to SGE. When SGE was treated with an excess of properdin, it was unable to inhibit the AP. Through SDS-PAGE analysis, A. aquasalis presented a salivary protein with the same molecular weight as recombinant complement inhibitors belonging to the SG7 family described in the saliva of other anopheline species. At least some SG7 proteins bind to properdin and are AP inhibitors. Searching for SG7 proteins in the A. aquasalis genome, we retrieved a salivary protein that shared an 85% identity with albicin, which is the salivary alternative pathway inhibitor from A. albimanus. This A. aquasalis sequence was also very similar (81% ID) to the SG7 protein from A. darlingi, which is also an AP inhibitor. Our results suggest that the salivary complement inhibitor from A. aquasalis is an SG7 protein that can inhibit the AP by binding to properdin and abrogating its stabilizing activity. Albicin, which is the SG7 from A. albimanus, can directly inhibit AP convertase. Given the high similarity of SG7 proteins, the SG7 from A. aquasalis may also directly inhibit AP convertase in the absence of properdin. | pt_BR |
| Affilliation | Universidade Federal do de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Maranhão. Programa de Pós-graduação em Ciências da Saúde. São Luís, MA, Brasil. | pt_BR |
| Affilliation | Universidade Federal do de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Molecular Biology Section. Rockville, MD, USA. | pt_BR |
| Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Molecular Biology Section. Rockville, MD, USA. | pt_BR |
| Affilliation | Universidade Federal do de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Anopheles aquasalis | pt_BR |
| Subject | Saliva | pt_BR |
| Subject | Complement system | pt_BR |
| Subject | Alternative pathway | pt_BR |
| Subject | Complement system inhibition | pt_BR |
| Subject | Hematophagy | pt_BR |
| Embargo date | 2024-01-01 | |
