| Author | Nuovo, Gerard J. | |
| Author | Andrade, Cecilia Vianna de | |
| Author | Thirukkumaran, Chandini | |
| Author | Nicol, Alcina F. | |
| Access date | 2018-03-22T17:33:43Z | |
| Available date | 2018-03-22T17:33:43Z | |
| Document date | 2018 | |
| Citation | NUOVO, Gerald J. et al. Importin-β and exportin-5 are indicators of acute viral infection: correlation of their detection with commercially available detection kits. Annals of Diagnostic Pathology, v. 34, p. 36-41, 2018. | pt_BR |
| ISSN | 1092-9134 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25469 | |
| Language | por | pt_BR |
| Publisher | Elsivier | pt_BR |
| Rights | restricted access | |
| Title | Importin-β and exportin-5 are indicators of acute viral infection: Correlation of their detection with commercially available detection kits | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.anndiagpath.2018.01.003 | |
| Abstract | This work focused on immunohistochemistry markers of acute viral infections. Viral infected cells were detected by in situ based methods (reovirus, rabies virus) or cytologic changes (human papillomavirus, molloscum contagiosum virus, herpes simplex virus). Two proteins involved in nuclear trafficking, importin-β and exportin5, were detected in the infected cells for each virus and not in the control tissues. A wide variety of other proteins, including caspase-3, and bcl-2 family members (bcl2, bclX, MCL1, BAK, BAX, BIM, BAD) showed wide variations in expression among the different viral infections. Specificity of the importin-β and exportin-5 signals varied greatly with different commercially available peroxidase conjugates. It is concluded that immunohistochemistry detection of importin-β and exportin-5 may be useful markers of acute viral infection, which suggests that increased nuclear trafficking may be an important concomitant of viral proliferation. | pt_BR |
| Affilliation | Ohio State University Comprehensive Cancer Center Columbus, OH. United States / Phylogeny Medical Laboratory, Powell, OH, United States. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Tom Baker Cancer Centre, University of Calgary. Calgary, Canada. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Subject | Virus | pt_BR |
| Subject | Importin | pt_BR |
| Subject | Exportin | pt_BR |
| Subject | Background | pt_BR |
| Subject | Horseradish Peroxidase | pt_BR |
| DeCS | Virus | pt_BR |
| DeCS | Horseradish Peroxidase | pt_BR |
