| Author | Tonini, Aline de Castro Zacche | |
| Author | Fonseca, Giuliana Schmidt França | |
| Author | Jesus, Laura Néspoli Nassar Pansini de | |
| Author | Barros, Geisa Baptista | |
| Author | Reis, Jordana Grazziela Alves Coelho dos | |
| Author | Béla, Samantha Ribeiro | |
| Author | Machado, Anderson Silva | |
| Author | Carneiro, Ana Carolina Aguiar Vasconcelos | |
| Author | Andrade, Gláucia Manzan Queiroz | |
| Author | Santos, Daniel Vitor Vasconcelos | |
| Author | Januário, José Nélio | |
| Author | Carvalho, Andréa Teixeira de | |
| Author | Vitor, Ricardo Wagner Almeida | |
| Author | Ferro, Eloísa Amália Vieira | |
| Author | Mineo, José Roberto | |
| Author | Martins Filho, Olindo Assis | |
| Author | Lemos, Elenice Moreira | |
| Author | UFMG Congenital Toxoplasmosis Brazilian Group | |
| Access date | 2018-03-23T18:24:17Z | |
| Available date | 2018-03-23T18:24:17Z | |
| Document date | 2017 | |
| Citation | TONINI, Aline de Castro Zacche et al. Establishing tools for early diagnosis of congenital toxoplasmosis: Flow cytometric IgG avidity assay as a confirmatory test for neonatal screening. J Immunol Methods. , v. 451, p. 37-47, 2017. | pt_BR |
| ISSN | 0022-1759 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25479 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | infecção congenital | pt_BR |
| Subject in Portuguese | citometria de fluxo | pt_BR |
| Subject in Portuguese | diagnostico sorologico | pt_BR |
| Title | Establishing tools for early diagnosis of congenital toxoplasmosis: Flow cytometric IgG avidity assay as a confirmatory test for neonatal screening. | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jim.2017.08.005 | |
| Abstract | The aim of this study was to evaluate the performance of conventional serology (Q-Preven™ and ELFAVIDAS™) and flow cytometry-based serologic tools for early serologic diagnosis of congenital toxoplasmosis. The study groups included prospectively confirmed cases of congenital toxoplasmosis (TOXO = 88) and age-matching non-infected controls (NI = 15).The results demonstrated that all samples tested positive/indeterminate for anti-T. gondii IgM screening at birth using air-dried whole blood samples. Serum samples collected at 30–45 days after birth tested positive for ELFAVIDAS™ IgG in both groups. While all NI tested negative for ELFAVIDAS™ IgM and IgA, only 78% and 36% of TOXO tested positive for IgM and IgA, respectively. Flow cytometry-based anti-T. gondii IgM, IgA and IgG reactivity displayed moderate performance with low sensitivity (47.6%, 72.6% and 75.0%, respectively). Regardless the remarkable specificity of IgG1, IgG2 and IgG3 subclasses for early diagnosis, weak or moderate specificity was observed (Se = 73.9%, 60.2% and 83.0%, respectively). The analysis of IgG avidity indices (AI) demonstrated the highest performance among the flow cytometry-based methods (Se = 96.6%; Sp = 93.3%), underscoring the low avidity index (AI < 60%) within TOXO (97.0%) in contrast with the high avidity index (AI > 60%) in NI (93%). Analysis of anti-T. gondii IgG and IgG3 reactivity for mother:infant paired samples may represent a relevant complementary tests for early diagnosis. In conclusion, a feasible high-standard algorithm (Accuracy = 97.1%) was proposed consisting of Q-Preven™ IgM screening at birth, followed by ELFAVIDAS™ IgM and flow cytometric IgG avidity analysis at 30–45 days after birth as a high performance tool for early serological diagnosis of congenital toxoplasmosis. | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Vitória, ES, Brazil | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Vitória, ES, Brazil | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Vitória, ES, Brazil | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Vitória, ES, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil/Núcleo de Ações e pesquisa em Apoio Diagnóstico. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Núcleo de Ações e pesquisa em Apoio Diagnóstico. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Uberlândia, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal do Espírito Santo. Vitória, ES, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil | pt_BR |
| Subject | Congenital T. gondii infection | pt_BR |
| Subject | Flow cytometry | pt_BR |
| Subject | Serological diagnosis | pt_BR |
| Subject | IgM screening, | pt_BR |
| Subject | IgG avidity | pt_BR |
| Embargo date | 2023-01-01 | |
