| Author | Aquino, Priscila F. | |
| Author | Fischer, Juliana S. G. | |
| Author | Ferreira, Ana G. C. Neves | |
| Author | Perales, Jonas | |
| Author | Domont, Gilberto B. | |
| Author | Araujo, Gabriel D. T. | |
| Author | Barbosa, Valmir C. | |
| Author | Viana, Jucilana | |
| Author | Chalub, Sidney R. S. | |
| Author | Souza, Antonia Q. Lima de | |
| Author | Carvalho, Maria G. C. | |
| Author | Souza, Afonso D. Leão de | |
| Author | Carvalho, Paulo C. | |
| Access date | 2018-03-27T15:55:29Z | |
| Available date | 2018-03-27T15:55:29Z | |
| Document date | 2012 | |
| Citation | AQUINO, Priscila F. et al. Are Gastric Cancer Resection Margin Proteomic Profiles More Similar to Those from Controls or Tumors?. J. Proteome Res. v.11, p.5836−5842, Nov. 2012. | pt_BR |
| ISSN | 1535-3893 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25523 | |
| Language | eng | pt_BR |
| Publisher | American Chemical Society | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Neoplasias Gástricas | pt_BR |
| Subject in Portuguese | proteômica de espingarda | pt_BR |
| Subject in Portuguese | Margens de Excisão | pt_BR |
| Subject in Portuguese | microambiente | pt_BR |
| Title | Are gastric cancer resection margin proteomic profiles more similar to those from controls or tumors? | pt_BR |
| Type | Article | |
| DOI | 10.1021/pr300612x | |
| Abstract | A strategy for treating cancer is to surgically remove the tumor together with a portion of apparently healthy tissue surrounding it, the so-called "resection margin", to minimize recurrence. Here, we investigate whether the proteomic profiles from biopsies of gastric cancer resection margins are indeed more similar to those from healthy tissue than from cancer biopsies. To this end, we analyzed biopsies using an offline MudPIT shotgun proteomic approach and performed label-free quantitation through a distributed normalized spectral abundance factor approach adapted for extracted ion chromatograms (XICs). A multidimensional scaling analysis revealed that each of those tissue-types is very distinct from each other. The resection margin presented several proteins previously correlated with cancer, but also other overexpressed proteins that may be related to tumor nourishment and metastasis, such as collagen alpha-1, ceruloplasmin, calpastatin, and E-cadherin. We argue that the resection margin plays a key role in Paget's "soil to seed" hypothesis, that is, that cancer cells require a special microenvironment to nourish and that understanding it could ultimately lead to more effective treatments. | pt_BR |
| Affilliation | Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Bioquímica. Unidade de Proteômica. Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Bioquímica. Unidade de Proteômica. Rede Proteômica do Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. COPPE. Programa de Engenharia de Sistemas e Computação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Amazonas. Departamento de Cirurgia Digestiva. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Departamento de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Subject | gastric cancer | pt_BR |
| Subject | shotgun proteomics | pt_BR |
| Subject | microenvironment | pt_BR |
| Subject | resection margin | pt_BR |
| e-ISSN | 1535-3907 | |
| Embargo date | 2030-01-01 | |
