| Author | Saraiva, Victor Barbosa | |
| Author | Silva, Leandro de Souza | |
| Author | Silva, Claudio Teixeira Ferreira da | |
| Author | Silva Filho, João Luiz | |
| Author | Ferreira, André Teixeira | |
| Author | Perales, Jonas | |
| Author | Souza, Mariana Conceição | |
| Author | Henriques, Maria das Graças | |
| Author | Neves, Celso Caruso | |
| Author | Pinheiro, Ana Acacia de Sá | |
| Access date | 2018-03-27T16:52:15Z | |
| Available date | 2018-03-27T16:52:15Z | |
| Document date | 2011 | |
| Citation | SARAIVA, Victor Barbosa et al. Impairment of the Plasmodium falciparum Erythrocytic Cycle Induced by Angiotensin Peptides. Plos One, v. 6, n. 2, e17174, p. 1-9, Feb. 2011. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25528 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Plasmodium falciparum | pt_BR |
| Subject in Portuguese | Peptídeos | pt_BR |
| Subject in Portuguese | Peptides da angiotensina | pt_BR |
| Subject in Portuguese | Malaria | pt_BR |
| Subject in Portuguese | Ciclo Eritrocítico | pt_BR |
| Subject in Portuguese | Plasmodium falciparum | pt_BR |
| Title | Impairment of the Plasmodium falciparum erythrocytic cycle induced by angiotensin peptides | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0017174 | |
| Abstract | Plasmodium falciparum causes the most serious complications of malaria and is a public health problem worldwide with over 2 million deaths each year. The erythrocyte invasion mechanisms by Plasmodium sp. have been well described, however the physiological aspects involving host components in this process are still poorly understood. Here, we provide evidence for the role of renin-angiotensin system (RAS) components in reducing erythrocyte invasion by P. falciparum. Angiotensin II (Ang II) reduced erythrocyte invasion in an enriched schizont culture of P. falciparum in a dose-dependent manner. Using mass spectroscopy, we showed that Ang II was metabolized by erythrocytes to Ang IV and Ang-(1-7). Parasite infection decreased Ang-(1-7) and completely abolished Ang IV formation. Similar to Ang II, Ang-(1-7) decreased the level of infection in an A779 (specific antagonist of Ang-(1-7) receptor, MAS)-sensitive manner. 10(-7) M PD123319, an AT(2) receptor antagonist, partially reversed the effects of Ang-(1-7) and Ang II. However, 10(-6) M losartan, an antagonist of the AT(1) receptor, had no effect. Gs protein is a crucial player in the Plasmodium falciparum blood cycle and angiotensin peptides can modulate protein kinase A (PKA) activity; 10(-8) M Ang II or 10(-8) M Ang-(1-7) inhibited this activity in erythrocytes by 60% and this effect was reversed by 10(-7) M A779. 10(-6) M dibutyryl-cAMP increased the level of infection and 10(-7) M PKA inhibitor decreased the level of infection by 30%. These results indicate that the effect of Ang-(1-7) on P. falciparum blood stage involves a MAS-mediated PKA inhibition. Our results indicate a crucial role for Ang II conversion into Ang-(1-7) in controlling the erythrocytic cycle of the malaria parasite, adding new functions to peptides initially described to be involved in the regulation of vascular tonus. | pt_BR |
| Affilliation | Instituto Federal de Educação, Ciência e Tecnologia Fluminense. Cabo Frio, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Rede Proteômica do Rio de Janeiro Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Rede Proteômica do Rio de Janeiro Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Conselho Nacional de Desenvolvimento Científico e Tecnológico / Ministério de Ciência e Tecnologia. Instituto Nacional de Ciência e Tecnologia em Biologia e Bioimagem. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Conselho Nacional de Desenvolvimento Científico e Tecnológico. Brasília, DF, Brasil / Ministério de Ciência e Tecnologia. Instituto Nacional para Pesquisa Translacional em Saúde e Ambiente na Região Amazônica. Brasília, DF, Brasil. | pt_BR |
| Subject | Plasmodium falciparum | pt_BR |
| Subject | Angiotensin Peptides | pt_BR |
| Subject | Erythrocytic Cycle | pt_BR |
| Subject | Malaria | pt_BR |
