| Author | Reis, Rodrigo Siqueira | |
| Author | Rocha, Surza Lucia Gonçalves da | |
| Author | Chapeaurouge, Donat Alexander | |
| Author | Domont, Gilberto Barbosa | |
| Author | Santa Anna, Lídia Maria Melo | |
| Author | Freire, Denise Maria Guimarães | |
| Author | Perales, Jonas | |
| Access date | 2018-04-03T15:39:19Z | |
| Available date | 2018-04-03T15:39:19Z | |
| Document date | 2010 | |
| Citation | REIS, Rodrigo Siqueira; et al. Effects of carbon and nitrogen sources on the proteome of Pseudomonas aeruginosa PA1 during rhamnolipid production. Process Biochemistry, v.45, p.1504–1510, 2010. | pt_BR |
| ISSN | 1359-5113 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25586 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Pseudomonas aeruginosa | pt_BR |
| Subject in Portuguese | Proteômica | pt_BR |
| Subject in Portuguese | Fonte de carbono e nitrogênio | pt_BR |
| Subject in Portuguese | Ramnolipídio | pt_BR |
| Subject in Portuguese | Biorremediação | pt_BR |
| Title | Effects of carbon and nitrogen sources on the proteome of Pseudomonas aeruginosa PA1 during rhamnolipid production | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.procbio.2010.05.032 | |
| Abstract | The PA1 strain of Pseudomonas aeruginosa isolated from oil waste produces rhamnolipid, a biodegradable
surfactant with applications in several industrial and environmental fields. The metabolic pathway and
genetic regulation of rhamnolipid production in P. aeruginosa are poorly understood. Herein, several proteins
directly or indirectly related to rhamnolipid production and their genetic regulations were identified
by comparative proteomic. We compared the proteome of P. aeruginosa PA1 after fermentation in two
different conditions of carbon and nitrogen sources: condition A allowed rhamnolipid production and
condition B prevented it. Protein extracts from cellular pellets were compared using 2D-PAGE stained
with colloidal Coomassie followed by MALDI-TOF/TOF mass spectrometry. We identified 21 differentially
expressed proteins, including those involved in secretion, quorum sensing, oxidative response and
metabolism. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | PETROBRAS. Centro de Desenvolvimento e Pesquisa Leopoldo Américo Miguez. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Pseudomonas aeruginosa | pt_BR |
| Subject | Rhamnolipid | pt_BR |
| Subject | Carbon and nitrogen source | pt_BR |
| Subject | Bioremediation | pt_BR |
| Subject | Proteomics | pt_BR |
| e-ISSN | 1873-3298 | |
| Embargo date | 2030-01-01 | |
