| Autor | Sousa, Juliana Ferreira de | |
| Autor | Torrieri, Raul | |
| Autor | Serafim, Rodolfo Bortolozo | |
| Autor | Di Cristofaro, Luis Fernando Macedo | |
| Autor | Escanfella, Fábio Dalbon | |
| Autor | Ribeiro, Rodrigo | |
| Autor | Zanette, Dalila Lucíola | |
| Autor | Larson, Maria Luisa Paçó | |
| Autor | Silva, Wilson Araujo da | |
| Autor | Tirapelli, Daniela Pretti da Cunha | |
| Autor | Neder, Luciano | |
| Autor | Carlotti, Carlos Gilberto | |
| Autor | Valente, Valeria | |
| Data de acesso | 2018-04-06T13:45:43Z | |
| Data de disponibilização | 2018-04-06T13:45:43Z | |
| Data do publicação | 2017 | |
| Citação | SOUSA, J. F. et al. Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients | pt_BR |
| ISSN | 1010-4283 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25652 | |
| Descrição | Zanette, Dalila Lucíola. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
| Fomento | Fundação de Amparo à Pesquisa do Estado de São Paulo (grant no. 2013/13465-1) and Programa de
Apoio ao Desenvolvimento Científico (grant no. 204/2012) from Faculty of Pharmaceutical Sciences of Araraquara. Center for Cell-Based Therapy (CEPID/FAPESP; grant no. 2013/08135-2). | pt_BR |
| Idioma | eng | pt_BR |
| Editor | Springer Verlag | pt_BR |
| Direito Autoral | open access | pt_BR |
| Palavras-chave | Reparo de DNA | pt_BR |
| Palavras-chave | Astrocitoma | pt_BR |
| Palavras-chave | Glioblastoma | pt_BR |
| Palavras-chave | Progressão tumoral | pt_BR |
| Palavras-chave | Instabilidade genômica | pt_BR |
| Título | Expression signatures of DNA repair genes correlate with survival prognosis of astrocytoma patients | pt_BR |
| Tipo do documento | Article | pt_BR |
| DOI | 10.1177/1010428317694552 | |
| Resumo em Inglês | Astrocytomas are the most common primary brain tumors. They are very resistant to therapies and usually progress rapidly to high-grade lesions. Here, we investigated the potential role of DNA repair genes in astrocytoma progression and resistance. To this aim, we performed a polymerase chain reaction array-based analysis focused on DNA repair genes and searched for correlations between expression patters and survival prognoses. We found 19 genes significantly altered. Combining these genes in all possible arrangements, we found 421 expression signatures strongly associated with poor survival. Importantly, five genes (DDB2, EXO1, NEIL3, BRCA2, and BRIP1) were independently correlated with worse prognoses, revealing single-gene signatures. Moreover, silencing of EXO1, which is remarkably overexpressed, promoted faster restoration of double-strand breaks, while NEIL3 knockdown, also highly overexpressed, caused an increment in DNA damage and cell death after irradiation of glioblastoma cells. These results disclose the importance of DNA repair pathways for the maintenance of genomic stability of high-grade astrocytomas and suggest that EXO1 and NEIL3 overexpression confers more efficiency for double-strand break repair and resistance to reactive oxygen species, respectively. Thereby, we highlight these two genes as potentially related with tumor aggressiveness and promising candidates as novel therapeutic targets. | pt_BR |
| Afiliação | University of São Paulo State. Faculty of Pharmaceutical Sciences of Araraquara. Department of Clinical Analysis. Araraquara, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo. Ribeirão Preto Medical School. Center for Medical Genomics of the Clinical Hospital. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo State. Faculty of Pharmaceutical Sciences of Araraquara. Department of Clinical Analysis. Araraquara, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo State. Faculty of Pharmaceutical Sciences of Araraquara. Department of Clinical Analysis. Araraquara, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Regional Blood Center of Ribeirão Preto. Center for Cell-Based Therapy. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo. Ribeirão Preto Medical School. Department of Genetics. Ribeirão Preto, SP, Brazil / Regional Blood Center of Ribeirão Preto. Center for Cell-Based Therapy. Ribeirão Preto, SP, Brazil / National Institute of Science and Technology in Stem cell and Cell Therapy. Ribeirão Preto, SP, Brazil / University of São Paulo. Center for Integrative Systems Biology. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo. Ribeirão Preto Medical School. Department of Surgery and Anatomy. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo. Ribeirão Preto Medical School. Department of Pathology. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo. Center for Integrative Systems Biology. Ribeirão Preto, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Department of Pathology. Ribeirão Preto, SP, Brazil | pt_BR |
| Afiliação | University of São Paulo State. Faculty of Pharmaceutical Sciences of Araraquara. Department of Clinical Analysis. Araraquara, SP, Brazil / University of São Paulo. Ribeirão Preto Medical School. Department of Cellular and Molecular Biology. Ribeirão Preto, SP, Brazil / University of São Paulo. Center for Integrative Systems Biology. Ribeirão Preto, SP, Brazil | pt_BR |
| Palavras-chave em inglês | DNA repair | pt_BR |
| Palavras-chave em inglês | Astrocytoma | pt_BR |
| Palavras-chave em inglês | Glioblastoma | pt_BR |
| Palavras-chave em inglês | Tumor progression | pt_BR |
| Palavras-chave em inglês | Genomic instability | pt_BR |
