| Author | Rocha, Sura Wanessa Santos | |
| Author | Silva, Bruna Santos | |
| Author | Gomes, Fabiana Oliveira dos Santos | |
| Author | Soares e Silva, Amanda Karolina | |
| Author | Raposo, Catarina | |
| Author | Barbosa, Karla Patrícia Sousa | |
| Author | Torres, Dilênia de Oliveira Cipriano | |
| Author | Santos, Ana Célia Oliveira dos | |
| Author | Peixoto, Christina Alves | |
| Access date | 2018-04-12T12:39:19Z | |
| Available date | 2018-04-12T12:39:19Z | |
| Document date | 2012 | |
| Citation | SANTOS ROCHA, S. W. et al. Effect of diethylcarbamazine on chronic hepatic inflammation induced by alcohol in C57BL/6 mice. European Journal of Pharmacology, v. 689, n. 1–3, p. 194–203, ago. 2012. | pt_BR |
| ISSN | 1879-0712 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25792 | |
| Sponsorship | Este estudo foi financiado pela Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), Centro de Pesquisas Aggeu Magalhães da Fundação Oswaldo Cruz em Recife, Brasil (CPqAM / FIOCRUZ), Centro de Tecnologia Estratégica no Nordeste (CETENE) e Instituto Nacional de Biologia Estrutural e Bioimagem (INBEB). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Dietilcarbamazina | pt_BR |
| Subject in Portuguese | Alcoolismo | pt_BR |
| Subject in Portuguese | Marcadores Inflamatórios | pt_BR |
| Subject in Portuguese | Lesão hepática | pt_BR |
| Title | Effect of diethylcarbamazine on chronic hepatic inflammation induced by alcohol in C57BL/6 mice | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.ejphar.2012.05.044 | |
| Abstract | Some pharmacological studies showed that diethylcarbamazine (DEC) interferes with the arachidonic acid metabolism, acting as an anti-inflammatory drug. The chronic alcohol consumption activates the hepatic inflammatory response associated to T-cell activation and overproduction of pro-inflammatory cytokines. The present work analyzed the anti-inflammatory effect of DEC on hepatic cells of alcoholic mice. Thirty-two male C57BL/6 mice were equally divided in the following groups: (a) control group (C), which received only water, (b) DEC-treated group, which received 50 mg/kg for 12 day (DEC50), (c) the alcoholic group (EtOH), submitted to only alcohol and (d) the alcohol-DEC treated group (EtOH50), submitted to alcohol plus DEC treatment after the induction of chronic alcoholism for 5 weeks. Biochemical analyses were performed and liver fragments were processed for light microscopy, transmission electron microscopy, immunohistochemical and western blot. The level of AST increased significantly in alcoholic group whereas a significant reduction of serum AST was detected in the EtOH50 group. Histological and ultrastructural analysis of alcoholic group showed evident hepatocellular damage, which was strikingly reduced in the alcoholic DEC-treated group. Immunohistochemistry results revealed highly expression of inflammatory markers as MDA, NF-κB, TNF-α, IL-6, VCAM and ICAM by the hepatic cells of the EtOH group; however no immunoreactivity for any of these cytokines was detected after DEC treatment. Western blot analyses showed increased MCP-1 and iNOS expression in EtOH group, which was significantly inhibited by DEC treatment. According to the present results, DEC can be a potential drug for the treatment of chronic inflammation induced by chronic alcoholism. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Centro de Tecnologia Estratégicas do Nordeste. Laboratório Microscopia e Microanálise. Recife, PE, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | a) Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil / Universidade de Pernambuco. Laboratório deAnálises Clínicas. Recife, PE, Brazil. | pt_BR |
| Affilliation | Universidade de Pernambuco. Instituto de Ciências Biológicas. Recife, PE, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil / Centro deTecnologia Estratégicas do Nordeste. Laboratório Microscopia e Microanálise. Recife, PE, Brazil. | pt_BR |
| Subject | Diethylcarbamazine | pt_BR |
| Subject | Alcoholism | pt_BR |
| Subject | Inflammatory markers | pt_BR |
| Subject | Hepatic injury | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Dietilcarbamazina / farmacologia | pt_BR |
| DeCS | Dietilcarbamazina / uso terapêutico | pt_BR |
| DeCS | Etanol / administração & dosagem | pt_BR |
| DeCS | Etanol / efeitos adversos | pt_BR |
| DeCS | Hepatite Alcoólica / quimioterapia | pt_BR |
| DeCS | Hepatite Alcoólica / metabolismo | pt_BR |
| DeCS | Hepatite Alcoólica / patologia | pt_BR |
| DeCS | Mediadores / antagonistas da inflamação e inibidores | pt_BR |
| DeCS | Mediadores da Inflamação / metabolismo | pt_BR |
| DeCS | Masculino | pt_BR |
| DeCS | Ratos | pt_BR |
| DeCS | Ratos, endogâmicos C57BL | pt_BR |
| DeCS | Resultado do tratamento | pt_BR |
| Embargo date | 2050-01-01 | |
