| Author | Pacheco, R. S. | |
| Author | Crimaldi Jr, G. | |
| Author | Momen, H. | |
| Author | Morel, Carlos Medicis | |
| Access date | 2014-12-10T10:29:33Z | pt_BR |
| Access date | 2018-04-12T16:59:08Z | |
| Available date | 2014-12-10T10:29:33Z | pt_BR |
| Available date | 2018-04-12T16:59:08Z | |
| Document date | 1990 | |
| Citation | PACHECO, R. S. et al. Population heterogeneity amomg clones of New World Leishmania species. Parasitology, v.100, p.393-398, 1990 | pt_BR |
| ISSN | 00311820 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25825 | |
| Language | por | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | DNA de Cinetoplasto | pt_BR |
| Subject in Portuguese | Clonagem de células | pt_BR |
| Title | Population hetereogeneity among clones of New World Leishmania species | pt_BR |
| Type | Article | |
| Abstract | Cell cloning techniques and schizodeme analysis were used to detect mixtures of subpopulations in Leishmania parasites,
isolated from humans and a reservoir host. Clones were obtained by plating promastigotes, at limited dilution, on solid
medium. The resultant colonies were analysed by the restriction profile of kinetoplast DNA minicircles (schizodeme
analysis). The efficiency of plating was around 40-60%, and subpopulations of different schizodemes, in stocks of
Leishmania isolated from 2 cases of human cutaneous leishmaniasis were detected. The presence of different schizodemes
in one isolate from a sylvatic animal suggested the possibility of a mixed natural infection. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | cell clonning | pt_BR |
| Subject | kDNA | pt_BR |
| Subject | schizodemes | pt_BR |
| DeCS | Leishmania | pt_BR |
| xmlui.metadata.dc.publisher.publisher | Cambridge University Press | pt_BR |
