| Author | Pigosso, Laurine Lacerda | |
| Author | Baeza, Lilian Cristiane | |
| Author | Tomazett, Mariana Vieira | |
| Author | Faleiro, Mariana Batista Rodrigues | |
| Author | Moura, Veridiana Maria Brianezi Dignani de | |
| Author | Bailão, Alexandre Melo | |
| Author | Borges, CL | |
| Author | Rocha, Juliana Alves Parente | |
| Author | Fernandes, Gabriel da Rocha | |
| Author | Gauthier, GM | |
| Author | Soares, Célia Maria de Almeida | |
| Access date | 2018-04-20T17:03:41Z | |
| Available date | 2018-04-20T17:03:41Z | |
| Document date | 2017 | |
| Citation | PIGOSSO, Laurine Lacerda et al. Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection. Virulence, v. 8, n. 7, p. 1417-1434, 2017. | pt_BR |
| ISSN | 2150-5594 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/25987 | |
| Language | eng | pt_BR |
| Publisher | Taylor & Francis | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Paracoccidioides brasiliensis | pt_BR |
| Title | Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection | pt_BR |
| Type | Article | |
| DOI | 10.1080/21505594.2017.1355660 | |
| Abstract | Paracoccidoides brasiliensis and Paracoccidioides lutzii, the etiologic agents of paracoccidioidomycosis, cause disease in healthy and immunocompromised persons in Latin America. We developed a method for harvesting P. brasiliensis yeast cells from infected murine lung to facilitate in vivo transcriptional and proteomic profiling. P. brasiliensis harvested at 6 h post-infection were analyzed using RNAseq and LC-MSE. In vivo yeast cells had 594 differentially expressed transcripts and 350 differentially expressed proteins. Integration of transcriptional and proteomic data indicated that early in infection (6 h), P. brasiliensis yeast cells underwent a shift in metabolism from glycolysis to β-oxidation, upregulated detoxifying enzymes to defend against oxidative stress, and repressed cell wall biosynthesis. Bioinformatics and functional analyses also demonstrated that a serine proteinase was upregulated and secreted in vivo. To our knowledge this is the first study depicting transcriptional and proteomic data of P. brasiliensis yeast cells upon 6 h post-infection of mouse lung. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Escola de Veterinária e Zootecnia. Laboratório de Patologia Goiânia, GO, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Escola de Veterinária e Zootecnia. Laboratório de Patologia Goiânia, GO, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG , Brazil. | pt_BR |
| Affilliation | University of Wisconsin. Department of Medicine . Madison , WI , USA. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brazil. | pt_BR |
| Subject | Paracoccidioides brasiliensis | pt_BR |
| Subject | metabolic adaptation | pt_BR |
| Subject | proteome | pt_BR |
| Subject | serine proteinase | pt_BR |
| Subject | transcriptome | pt_BR |
| Embargo date | 2022-01-01 | |
