| Author | Tomazett, Patrícia Kott | |
| Author | Félix, Carlos Roberto | |
| Author | Lenzi, Henrique Leonel | |
| Author | Faria, Fabrícia de Paula | |
| Author | Soares, Célia Maria de Almeida | |
| Author | Pereira, Maristela | |
| Access date | 2018-04-24T16:22:51Z | |
| Available date | 2018-04-24T16:22:51Z | |
| Document date | 2010 | |
| Citation | TOMAZETT, Patrícia Kott et al. 1,3-b-D-Glucan synthase of Paracoccidioides brasiliensis: recombinant protein, expression and cytolocalization in the yeast and mycelium phases. Fungal Biology, v. 114, p. 809-816, 2010. | pt_BR |
| ISSN | 1878-6146 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26068 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Parede celular | pt_BR |
| Subject in Portuguese | Paracoccidioides | pt_BR |
| Subject in Portuguese | Proteína recombinante | pt_BR |
| Subject in Portuguese | Citolocalização | pt_BR |
| Title | 1,3-β-d-Glucan synthase of Paracoccidioides brasiliensis: recombinant protein, expression and cytolocalization in the yeast and mycelium phases | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.funbio.2010.07.007 | |
| Abstract | Paracoccidioides brasiliensis is a thermo-dimorphic human pathogenic fungus that in the mycelium phase lives at 23°C in environment and in the yeast phase at 37°C in the host tissues. In P. brasiliensis, the main polymers that compound the cell wall are chitin, 1,3-β-D-glucan and 1,3-α-glucan. They make a primary barrier responsible for the structural integrity and form of the cell wall. In P. brasiliensis, just one homologue of 1,3-β-D-glucan synthase gene (PbFKS1) was found. Here, the active recombinant protein (PbFks1pc) containing the catalytic region was obtained in Escherichia coli. In addition, a paradoxical dissociation was detected between the expression of the PbFKS1 transcript and the level of the corresponding protein PbFks1p, which was higher in the yeast phase, versus the amount of 1,3-β-D-glucan polymer, which was higher in the mycelium phase. Western blot analysis using protein extracts of cellular fractions showed that PbFks1p is present in the membrane-enriched fraction of mycelium and yeast cells and in the cell wall-enriched fractions of yeast cells. Confocal-immunocytolocalization of PbFks1p identified the protein in the apical growing region of the mycelium and distributed on the surface of the yeast cell. Two possible mechanisms could explain the above-mentioned discrepancy between the data: (a) overexpression of Rho1 GTPase as a regulator of 1,3-β-D-glucan synthase; (b) possible post-translational regulation of PbFks1p in P. brasiliensis isolates. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular. Goiânia, GO, Brasil. | pt_BR |
| Affilliation | Universidade de Brasília. Instituto de Biologia. Laboratório de Enzimologia. Brasília, DF, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular. Goiânia, GO, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular. Goiânia, GO, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Goiás. Instituto de Ciências Biológicas. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular. Goiânia, GO, Brasil. | pt_BR |
| Subject | Cell wall | pt_BR |
| Subject | Cytolocalization | pt_BR |
| Subject | Paracoccidioides brasiliensis | pt_BR |
| Subject | Recombinant protein | pt_BR |
| Embargo date | 2030-12-31 | |
