| Author | Sardella, Isabela Gama | |
| Author | Singh, Mahavir | |
| Author | Kumpfer, Susanne | |
| Author | Heringer, Rafael Ribeiro | |
| Author | Saad, Maria Helena Féres | |
| Author | Sohler, Marzia Puccioni | |
| Access date | 2018-04-26T14:53:33Z | |
| Available date | 2018-04-26T14:53:33Z | |
| Document date | 2010 | |
| Citation | SARDELLA, Isabela Gama; et al. Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients. Mem Inst Oswaldo Cruz, v.105, n.5, p. 722-728, Aug. 2010. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26134 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Tuberculose | pt_BR |
| Subject in Portuguese | Líquido Cefalorraquidiano | pt_BR |
| Subject in Portuguese | Ensaio de Imunoadsorção Enzimática | pt_BR |
| Subject in Portuguese | Mycobacterium tuberculosis | pt_BR |
| Subject in Portuguese | Resposta imune | pt_BR |
| Title | Evaluation of Lionex TB kits and mycobacterial antigens for IgG and IgA detection in cerebrospinal fluid from tuberculosis meningitis patients | pt_BR |
| Type | Article | |
| Abstract | To evaluate commercial Lionex TB together with four antigens of Mycobacterium tuberculosis (MPT-64, MT10.3, 16 kDa and 38 kDa) for IgG and IgA cerebrospinal fluid (CSF) detection in the diagnosis of tuberculosis meningitis (TBM) with CSF negative acid-fast bacilli staining, 19 cases of TBM, 64 cases of other infectious meningoencephalitis and 73 cases of other neurological disorders were tested by enzyme linked immunosorbent assay. IgA-MPT-64 and IgG Lionex showed the highest sensitivities, specificities, positive predictive value and negative predictive value (63.2%, 47.4%; 95%, 93.7%; 40%, 98% and 28.4%, 97.1%, respectively). However, while grey zone was 12.7% and 6%, respectively, lowering sensitivity but maintains high specificity (≥ 95%). High protein concentration in CSF was associated with antibody positivity CSF/HIV+ which did not influence the sensitivity of both tests. To our knowledge, this is the first description of IgA-MPT-64 and IgG Lionex antibodies in CSF-TBM and, although there is good specificity, adjustments are needed based on antigen composition to enhance sensitivity. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Lionex Diagnostics and Therapeutics. Braunschweig, Germany. | pt_BR |
| Affilliation | Lionex Diagnostics and Therapeutics. Braunschweig, Germany. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Laboratório Neurolife. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Laboratório Neurolife. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | tuberculosis | pt_BR |
| Subject | cerebrospinal fluid | pt_BR |
| Subject | ELISA | pt_BR |
| Subject | meningoencephalitis tuberculosis | pt_BR |
| Subject | Mycobacterium tuberculosis | pt_BR |
| Subject | immune response | pt_BR |
| e-ISSN | 1678-8060 | |
