| Author | Henriques, Cristina | |
| Author | Moreira, Thiago Luiz B. | |
| Author | Brigagão, Claudia Maia | |
| Author | Pons, Andréa Henriques | |
| Author | Carvalho, Técia Maria U. | |
| Author | Souza, Wanderley de | |
| Access date | 2018-04-26T16:26:31Z | |
| Available date | 2018-04-26T16:26:31Z | |
| Document date | 2011 | |
| Citation | HENRIQUES, Cristina et al. Tetrazolium salt based methods for high-throughput evaluation of anti-parasite chemotherapy. Analytical Methods, v. 3, p. 2148-2155, 2011. | pt_BR |
| ISSN | 1759-9660 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26144 | |
| Language | eng | pt_BR |
| Publisher | Royal Society of Chemistry | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Sais de Tetrazólio | pt_BR |
| Subject in Portuguese | quimioterapia anti-parasita | pt_BR |
| Subject in Portuguese | Métodos de avaliação | pt_BR |
| Title | Tetrazolium salt based methods for high-throughput evaluation of anti-parasite chemotherapy | pt_BR |
| Type | Article | |
| DOI | 10.1039/c1ay05219e | |
| Abstract | This study demonstrates the first standardization of the MTS/PMS tetrazolium-based method in T.
cruzi and G. duodenalis, and its application for chemotherapy studies and drug screening. We improved
the detection limit and compared the MTT, XTT/PMS and MTS/PMS methods, in T. cruzi
epimastigotes, demonstrating that MTS/PMS is more reproducible, sensitive, faster and easier to
manipulate than XTT/PMS and MTT. Oligomycin (10 mg ml 1) reduced T. cruzi epimastigote
metabolic activity by 60% measured by the MTS/PMS method, which is in accordance with flow
cytometry measurements. To validate the MTS/PMS method, an IC50 of 10.5 mMwas estimated for the
drug amiodarone in T. cruzi and an IC50 of 6.2 mM for metronidazole in G. duodenalis, corroborating
the IC50 values found in the literature. In G. duodenalis, a protozoan that has remnant mitochondria,
the MTS/PMS method displayed higher sensitivity than T. cruzi epimastigotes. The detection limit was
104 for G. duodenalis trophozoites and 2.5 105 for T. cruzi epimastigotes. The intensity of the colored
MTS/PMS formazan derivative is proportional to the number of metabolically active protozoa,
regardless of whether used in a mitochondrial or an ‘‘amitochondrial’’ organism. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Bertha Meyer. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Bertha Meyer. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Bertha Meyer. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Bertha Meyer. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Bertha Meyer. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | chemotherapy. | pt_BR |
| Subject | anti-parasite | pt_BR |
| Subject | Tetrazolium | pt_BR |
| Subject | high-throughput evaluation | pt_BR |
| e-ISSN | 1759-9679 | |
| Embargo date | 2030-01-01 | |
