| Author | Rasinhas, Arthur da Costa | |
| Author | Silva, Marcos Alexandre Nunes da | |
| Author | Caldas, Gabriela Cardoso | |
| Author | Jácome, Fernanda Cunha | |
| Author | Leonardo, Raphael | |
| Author | Santos, Flávia Barreto dos | |
| Author | Nunes, Priscila Conrado Guerra | |
| Author | Barth, Ortrud Monika | |
| Author | Vieira, Debora Ferreira Barreto | |
| Access date | 2018-05-03T12:03:04Z | |
| Available date | 2018-05-03T12:03:04Z | |
| Document date | 2018 | |
| Citation | RASINHAS, Arthur da Costa; et al. First detection of dengue virus in the saliva of immunocompetent murine model. Mem Inst Oswaldo Cruz, v.113, n.4,: e170208, 2018. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26225 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Vírus da Dengue tipo 4 | pt_BR |
| Subject in Portuguese | modelo murino imunocompetente | pt_BR |
| Subject in Portuguese | saliva | pt_BR |
| Title | First detection of dengue virus in the saliva of immunocompetent murine model | pt_BR |
| Type | Article | |
| DOI | 10.1590/0074-02760170208 | |
| Abstract | The lack of an experimental animal model for the study of dengue pathogenesis is a limiting factor for the development
of vaccines and drugs. In previous studies, our group demonstrated the susceptibility of BALB/c mice to infection
by dengue virus (DENV) 1 and 2, and the virus was successfully isolated in several organs. In this study, BALB/c
mice were experimentally infected intravenously with DENV-4, and samples of their saliva were collected. Viral RNA
extracted from the saliva samples was subjected to qRT-PCR, with a detection limit of 0.002 PFU/mL. The presence
of DENV-4 viral RNA was detected in the saliva of two mice, presenting viral titers of 109 RNA/mL. The detection of
DENV RNA via saliva sampling is not a common practice in dengue diagnosis, due to the lower detection rates in human
patients. However, the results observed in this study seem to indicate that, as in humans, detection rates of DENV
RNA in mouse saliva are also low, correlating the infection in both cases. This study reports the first DENV detection
in the saliva of BALB/c immunocompetent mice experimentally infected with non-neuroadapted DENV-4. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | dengue 4 | pt_BR |
| Subject | saliva | pt_BR |
| Subject | BALB/c mice | pt_BR |
| Subject | immunocompetent murine model | pt_BR |
| e-ISSN | 1678-8060 | |
