| Author | Amorim, Liliane Barbosa | |
| Author | Barros, Rosineide Arruda de | |
| Author | Chalegre, Karlos Diogo de Melo | |
| Author | Oliveira, Cláudia Maria Fontes de | |
| Author | Regis, Lêda Narcisa | |
| Author | Silva-Filha, Maria Helena Neves Lobo | |
| Access date | 2018-05-03T14:22:30Z | |
| Available date | 2018-05-03T14:22:30Z | |
| Document date | 2010 | |
| Citation | AMORIM, L. B. et al. Stability of Culex quinquefasciatus resistance to Bacillus sphaericus evaluated by molecular tools. Insect Biochemistry and Molecular Biology, v. 40, n. 4, p. 311–316, abr. 2010. | pt_BR |
| ISSN | 1879-0240 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26239 | |
| Sponsorship | Este trabalho contou com o apoio do Conselho Nacional de Pesquisa (CNPq Brasil, bolsa 471911 / 2006-2 ) e Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE Brasil, outorga APQ 0060-2.02 / 07 ), Fundação Oswaldo Cruz (FIOCRUZ). Brasil, concessão PAPES IV 400336 / 2006-5). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Toxina binária | pt_BR |
| Subject in Portuguese | Cqm1 | pt_BR |
| Subject in Portuguese | α-Glucosidade | pt_BR |
| Subject in Portuguese | Alelo de resistência | pt_BR |
| Subject in Portuguese | PCR | pt_BR |
| Subject in Portuguese | Custo de fitness | pt_BR |
| Title | Stability of Culex quinquefasciatus resistance to Bacillus sphaericus evaluated by molecular tools | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.ibmb.2010.02.002 | |
| Abstract | Bacillus sphaericus binary toxin action on Culex quinquefasciatus larvae relies on the binding to Cqm1alpha-glucosidases, which act as midgut receptors. Resistance of two laboratory-selected colonies is associated with the allele cqm1(REC) that prevents Cqm1 expression as membrane-bound molecules. This study evaluated stability of resistance after the interruption of selection pressure and introduction of susceptible individuals in these colonies. Bioassays showed that frequency of resistant larvae did not decrease throughout 11 generations, under these conditions, and it was associated to a similar frequency of larvae lacking the Cqm1alpha-glucosidase receptor, detected by in gel enzymatic assays. Direct screening of the cqm1(REC) allele, by specific PCR, showed that its frequency remained stable throughout 11 generations. Parental resistant colony did not display biological costs regarding fecundity, fertility and pupal weight and data from susceptibility assays, enzymatic assays and PCR screening showed that cqm1(REC) was not disfavored in competition with the susceptible allele and persisted in the progenies, in the lack of selection pressure. Characterization of molecular basis of resistance is essential for developing diagnostic tools and data have relevant implication for the establishment of strategies for resistance management. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. | pt_BR |
| Subject | Binary toxin | pt_BR |
| Subject | Cqm1 | pt_BR |
| Subject | α-Glucosidade | pt_BR |
| Subject | Resistance allele | pt_BR |
| Subject | PCR | pt_BR |
| Subject | Fitness cost | pt_BR |
| DeCS | Alelos | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Bacilo / fisiologia | pt_BR |
| DeCS | Culex / genética | pt_BR |
| DeCS | Culex / imunologia | pt_BR |
| DeCS | Culex / microbiologia | pt_BR |
| DeCS | Fêmea | pt_BR |
| DeCS | Interações Host-Pathogen | pt_BR |
| DeCS | Imunidade Inata | pt_BR |
| DeCS | Proteínas de Insetos / genética | pt_BR |
| DeCS | Masculino | pt_BR |
| DeCS | Reação em Cadeia da Polimerase | pt_BR |
| DeCS | alfa-glucosidases / genética | pt_BR |
| Embargo date | 2050-01-01 | |
