| Author | Pereira, Marcela Araújo | |
| Author | Rebouças, Juliana de Souza | |
| Author | Carvalho, Rafaela de Siqueira Ferraz | |
| Author | Redín, Inés Luis de | |
| Author | Guerra, Priscila Valera | |
| Author | Gamazo, Carlos | |
| Author | Brodskyn, Claudia Ida | |
| Author | Irache, Juan M | |
| Author | Magalhães, Nereide Stela Santos | |
| Access date | 2018-05-10T17:27:05Z | |
| Available date | 2018-05-10T17:27:05Z | |
| Document date | 2018 | |
| Citation | PEREIRA, M. A. et al. Poly(anhydride) nanoparticles containing cashew nut proteins can induce a strong Th1 and Treg immune response after oral administration. European Journal of Pharmaceutics and Biopharmaceutics, v. 127, p. 51–60, 2018. | pt_BR |
| ISSN | 0939-6411 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26398 | |
| Sponsorship | Brazilian Ministry of Science and Technology – MCTI (SisNANO/LARNano-UFPE, CNPq # 402282/2013-2) and FACEPE (APQ #0361-4.03/14). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Nanopartículas | pt_BR |
| Subject in Portuguese | Alergia alimentar | pt_BR |
| Subject in Portuguese | Castanha de caju | pt_BR |
| Subject in Portuguese | Orais | pt_BR |
| Subject in Portuguese | Imunização | pt_BR |
| Title | Poly(anhydride) nanoparticles containing cashew nut proteins can induce a strong Th1 and Treg immune response after oral administration | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.ejpb.2018.02.011 | |
| Abstract | Cashew nut allergy is the second most commonly reported tree nut allergy. Traditional allergen immunotherapy presents several clinical drawbacks that can be reduced by using nanoparticles-basedallergen-delivery systems, modulating the immune response towards a protective one. In this context, the goal of this work was to assess the potential of poly(anhydride) nanoparticles (NP) for cashew nut oral immunization. Cashew nut allergens-loaded nanoparticles (CNE-NP) were prepared by solvent displacement method. After nanoparticles characterization, oral immunomodulation ability was evaluated in BALB/c mice. Our results demonstrated that CNE-NP induced a higher Th1/Th2 ratio in comparison with animals immunized with free cashew nut proteins. Indeed, a decrease in splenic Th2 cytokines (IL-4, IL-5, and IL-13), and an enhancement of pro-Th1 (IL-12 and IFN-γ) and regulatory (IL-10) cytokines was observed. Furthermore, mice orally immunized with CNE-NP presented an increased expansion of CD4+ T regulatory cells, such as CD4+Foxp3+ and CD4+LAP+, in the mesenteric lymph nodes. In conclusion, oral immunization with a single dose of poly(anhydride) nanoparticles loaded with cashew nut proteins leaded to a pro-Th1 and Treg immune response. Furthermore, their immunomodulatory properties could be introduced as a new approach for management of cashew nut allergy. | pt_BR |
| Affilliation | Federal University of Pernambuco. Immunopathology Keizo-Asami Laboratory. Recife, PE, Brazil | pt_BR |
| Affilliation | University of Pernambuco. Institute of Biological Sciences. Recife, PE, Brazil | pt_BR |
| Affilliation | Federal University of Pernambuco. Immunopathology Keizo-Asami Laboratory. Recife, PE, Brazil | pt_BR |
| Affilliation | University of Navarra. Nanomedicines and Vaccines. Research Group. Pamplona, Spain | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Parasita-Hospedeiro Interação e Epidemiologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of Navarra. Nanomedicines and Vaccines. Research Group. Pamplona, Spain | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Parasita-Hospedeiro Interação e Epidemiologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of Navarra. Nanomedicines and Vaccines. Research Group. Pamplona, Spain | pt_BR |
| Affilliation | Federal University of Pernambuco. Immunopathology Keizo-Asami Laboratory. Recife, PE, Brazil | pt_BR |
| Subject | Nanoparticles | pt_BR |
| Subject | Food allergy | pt_BR |
| Subject | Cashew nut | pt_BR |
| Subject | Oral | pt_BR |
| Subject | Immunization | pt_BR |
| xmlui.metadata.dc.subject.ods | 02 Fome zero e agricultura sustentável | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
